Nowinski R C, Edynak E, Sarkar N H
Proc Natl Acad Sci U S A. 1971 Jul;68(7):1608-12. doi: 10.1073/pnas.68.7.1608.
We describe here serological and structural properties of a virus, Mason-Pfizer Monkey Virus (M-PMV), isolated from a simian mammary tumor. This virus is morphologically similar to the known oncogenic RNA viruses (oncornaviruses). It has a 60-70S RNA, and its replication is inhibited by actinomycin. Antisera prepared against the virus isolated by density-gradient centrifugation identify at least two viral structural antigens. Immunodiffusion studies show that this virus is serologically unrelated to three types of simian foamy viruses, visna virus, and the known oncornaviruses. Immunofluorescence reveals that the structural proteins of the virus are synthesized cytoplasmically. Although M-PMV productively infects human cells in vitro, serological analysis does not show the presence of M-PMV antigens in human neoplasia.
我们在此描述从一只猿猴乳腺肿瘤中分离出的一种病毒——梅森 - 辉瑞猴病毒(M-PMV)的血清学和结构特性。这种病毒在形态上与已知的致癌RNA病毒(肿瘤病毒)相似。它有一个60 - 70S的RNA,其复制受到放线菌素的抑制。用密度梯度离心法分离出的病毒制备的抗血清可识别至少两种病毒结构抗原。免疫扩散研究表明,这种病毒在血清学上与三种猿猴泡沫病毒、维斯纳病毒以及已知的肿瘤病毒无关。免疫荧光显示该病毒的结构蛋白在细胞质中合成。尽管M-PMV在体外能有效感染人类细胞,但血清学分析并未显示人类肿瘤中存在M-PMV抗原。