梅森- Pfizer猴病毒两种多肽的免疫学特性
Immunological properties of two polypeptides of Mason-Pfizer monkey virus.
作者信息
Tronick S R, Stephenson J R, Aaronson S A
出版信息
J Virol. 1974 Jul;14(1):125-32. doi: 10.1128/JVI.14.1.125-132.1974.
Abstract
Two polypeptides were isolated from the Mason-Pfizer monkey virus (MP-MV). The polypeptides, designated MP-MV p26 and MP-MV p15, were 26,000 and 15,000 molecular weight, respectively, based on gel filtration chromatography in the presence of 6 M guanidine hydrochloride. Radioimmunoassays developed for MP-MV p26 and p15 were, respectively, 10 and 100 times more sensitive than immunodiffusion and 1 and 10 times more sensitive than micro-complement fixation for the detection of MP-MV. Antigens of other reverse transcriptase-containing RNA viruses did not cross-react in either MP-MV radioimmunoassay. Further, antisera against these other viruses did not react with the radioiodine-labeled MP-MV polypeptides. The MP-MV radioimmunoassays should be useful in studying the natural occurrence of this virus and its relationship to primate tumors.
摘要
从梅森 - 辉瑞猴病毒（MP - MV）中分离出了两种多肽。这两种多肽分别命名为MP - MV p26和MP - MV p15，在6 M盐酸胍存在下通过凝胶过滤色谱法测定，其分子量分别为26,000和15,000。针对MP - MV p26和p15开发的放射免疫测定法，在检测MP - MV时，分别比免疫扩散法敏感10倍和100倍，比微量补体结合法敏感1倍和10倍。其他含逆转录酶的RNA病毒的抗原在任何一种MP - MV放射免疫测定中均无交叉反应。此外，针对这些其他病毒的抗血清与放射性碘标记的MP - MV多肽不发生反应。MP - MV放射免疫测定法在研究这种病毒的自然发生及其与灵长类肿瘤的关系方面应会很有用。
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