Tedesco F, Lachmann P J
Clin Exp Immunol. 1971 Sep;9(3):359-70.
C6 quantitation was carried out in rabbit and human sera by the single radial immunodiffusion technique. The C6 content of the rabbit and human sera used as standards was estimated by precipitin analysis, using an anti-C6 antiserum labelled with I. The mean C6 level in normal human serum was 11 μg/ml, whereas in normal rabbit serum it was 35 μg/ml. Sera from forty rabbits heterozygous for C6 deficiency were found to have a mean concentration of C6 of 14 μg/ml. The C6 level was estimated in sera from patients with various immunological disorders and found to be significantly higher in the sera from patients with rheumatoid arthritis and normal in the sera from patients with SLE, glomerulonephritis, nephrotic syndrome and myeloma. C6 haemolytic assays were found to correlate well with the antigenic assays only in fresh sera. In various circumstances this correlation breaks down, presumably because of C6 inactivator. This inactivator, in contrast to C3-inactivator, appears to be bound to antigen–antibody complement complexes.
采用单向辐射免疫扩散技术对兔血清和人血清中的C6进行定量分析。用作标准的兔血清和人血清中的C6含量通过沉淀素分析进行估算，使用用碘标记的抗C6抗血清。正常人血清中C6的平均水平为11μg/ml，而正常兔血清中为35μg/ml。发现40只C6缺乏杂合子兔的血清中C6的平均浓度为14μg/ml。对患有各种免疫疾病患者的血清中的C6水平进行了估算，发现类风湿性关节炎患者血清中的C6水平显著升高，而系统性红斑狼疮、肾小球肾炎、肾病综合征和骨髓瘤患者血清中的C6水平正常。仅在新鲜血清中发现C6溶血试验与抗原试验相关性良好。在各种情况下，这种相关性会失效，可能是由于C6灭活剂所致。与C3灭活剂不同，这种灭活剂似乎与抗原-抗体补体复合物结合。
