Agnello V, Winchester R J, Kunkel H G
Immunology. 1970 Dec;19(6):909-19.
A gel diffusion method for demonstrating precipitin reactions of C1q with aggregated γ-globulin and immune complexes is described. Optimal precipitin lines were found to occur with 0.6 per cent agarose in 0.01 M EDTA at pH 7.2 and ionic strength 0.1. Reduction and alkylation of γ-globulin aggregates destroyed the precipitability with C1q. Precipitation occurred only with aggregates greater than 19S and with soluble immune complexes formed in two to twenty times antigen excess. γ-Globulin complexes were detected by this procedure in hypocomplementaemic sera from patients with systemic lupus erythematosus and hypocomplementaemic joint fluids from patients with rheumatoid arthritis. The relevance of this system to complement consumption in various disease states is discussed.
本文描述了一种凝胶扩散法，用于证明C1q与聚集的γ-球蛋白及免疫复合物的沉淀素反应。发现在pH 7.2、离子强度0.1的0.01 M乙二胺四乙酸（EDTA）中含0.6%琼脂糖时可出现最佳沉淀素线。γ-球蛋白聚集体的还原和烷基化破坏了其与C1q的沉淀性。仅大于19S的聚集体以及在抗原过量2至20倍时形成的可溶性免疫复合物会发生沉淀。通过该方法在系统性红斑狼疮患者的低补体血症血清和类风湿性关节炎患者的低补体血症关节液中检测到了γ-球蛋白复合物。讨论了该系统与各种疾病状态下补体消耗的相关性。
