Kilian M, Roland K, Mestecky J
Infect Immun. 1981 Mar;31(3):942-51. doi: 10.1128/iai.31.3.942-951.1981.
The potential of secretory immunoglobulin A (S-IgA) to interfere with the initial phase of dental plaque formation was studied by using an in vitro method which permits the quantitative determination of the sorption of radiolabeled oral bacterial cells to hydroxyapatite (HA) beads. The importance of specific S-IgA antibodies was evaluated by a comparison of the effect of pure preparations of colostral S-IgA, polymeric myeloma IgA, or preabsorbed S-IgA. Specific antibody molecules bound at the HA surface significantly enhanced the sorption of two Streptococcus sanguis strains. In contrast, HA-bound S-IgA antibodies inhibited the sorption of Streptococcus mitior and Streptococcus salivarius. The same was true for Streptococcus mutans cells, but only when they were propagated in the absence of sucrose. Suspended in saliva, cells of all streptococcal species adhered in significantly lower numbers to HA. Comparative experiments with bacteria suspended in solutions of various preparations of IgA or immunoglobulin-deficient salivas with S-IgA or myeloma IgA added indicated that the adherence inhibition seen with S. Sanguis, S. mitior, S. salivarius, and glucose-grown S. mutans was partly attributable to functions of S-IgA antibodies. Under the in vitro conditions of the study, S-IgA antibodies had no effect on the sorption of sucrose-grown S. mutans, Actinomyces viscosus, and Actinomyces naeslundii to HA. The results indicated that S-IgA can interfere with the sorption of some oral bacteria to HA by several different functions.
采用一种体外方法研究了分泌型免疫球蛋白A（S-IgA）干扰牙菌斑形成初始阶段的潜力，该方法能够定量测定放射性标记的口腔细菌细胞对羟基磷灰石（HA）珠的吸附。通过比较初乳S-IgA纯制剂、聚合性骨髓瘤IgA或预吸收S-IgA的效果，评估了特异性S-IgA抗体的重要性。结合在HA表面的特异性抗体分子显著增强了两种血链球菌菌株的吸附。相比之下，结合在HA上的S-IgA抗体抑制了轻链球菌和唾液链球菌的吸附。变形链球菌细胞也是如此，但前提是它们在无糖条件下繁殖。悬浮在唾液中时，所有链球菌属的细胞对HA的黏附数量均显著降低。用悬浮在添加了S-IgA或骨髓瘤IgA的各种IgA制剂溶液或免疫球蛋白缺陷唾液中的细菌进行的对比实验表明，血链球菌、轻链球菌、唾液链球菌和葡萄糖培养的变形链球菌所出现的黏附抑制部分归因于S-IgA抗体的功能。在该研究的体外条件下，S-IgA抗体对蔗糖培养的变形链球菌、黏性放线菌和内氏放线菌吸附到HA上没有影响。结果表明，S-IgA可通过几种不同功能干扰某些口腔细菌对HA的吸附。
