Belas M R, Colwell R R
J Bacteriol. 1982 Sep;151(3):1568-80. doi: 10.1128/jb.151.3.1568-1580.1982.
The adsorption of laterally and polarly flagellated bacteria to chitin was measured, and from the data obtained, a modified Langmuir adsorption isotherm was derived. Results indicated that the adsorption of laterally flagellated Vibrio parahaemolyticus follows the Langmuir adsorption isotherm, a type of adsorption referred to as surface saturation kinetics, when conditions are favorable for the production of lateral flagella. When conditions were not favorable for the production of lateral flagella, bacterial adsorption did not follow the Langmuir adsorption isotherm; instead, proportional adsorption kinetics were observed. The adsorption of some polarly flagellated bacteria exhibited surface saturation kinetics. However, the binding index (the product of the number of binding sites and bacterial affinity to the surface) of polarly flagellated bacteria differed significantly from that of laterally flagellated bacteria, suggesting that polarly flagellated bacteria adsorb to chitin by a different mechanism from that used by the laterally flagellated bacteria. From the results of dual-label adsorption competition experiments, in which polarly flagellated V. cholerae competed with increasing concentrations of laterally flagellated V. parahaemolyticus, it was observed that laterally flagellated bacteria inhibited the adsorption of polarly flagellated bacteria. In contrast, polarly flagellated bacteria enhanced the adsorption of V. cholerae. In competition experiments, where V. parahaemolyticus competed against increasing concentrations of other bacteria, polarly flagellated bacteria enhanced V. parahaemolyticus adsorption significantly, whereas laterally flagellated bacteria only slightly enhanced the process. The direct correlation observed between surface saturation kinetics, the production of lateral flagella, and the ability of laterally flagellated bacteria to inhibit the adsorption of polarly flagellated bacteria suggests that lateral flagella represent a component of bacterial structure that is important in the adsorption of laterally flagellated bacteria to surfaces. A model for adsorption events of laterally flagellated bacteria is proposed, based on the evidence presented.
对具有侧生鞭毛和极生鞭毛的细菌吸附几丁质的情况进行了测定，并根据所得数据推导了修正的朗缪尔吸附等温线。结果表明，当条件有利于侧生鞭毛产生时，具有侧生鞭毛的副溶血性弧菌的吸附遵循朗缪尔吸附等温线，这种吸附类型被称为表面饱和动力学。当条件不利于侧生鞭毛产生时，细菌吸附不遵循朗缪尔吸附等温线；相反，观察到的是比例吸附动力学。一些具有极生鞭毛的细菌的吸附表现出表面饱和动力学。然而，具有极生鞭毛的细菌的结合指数（结合位点数与细菌对表面亲和力的乘积）与具有侧生鞭毛的细菌有显著差异，这表明具有极生鞭毛的细菌以与具有侧生鞭毛的细菌不同的机制吸附到几丁质上。从双标记吸附竞争实验的结果来看，在该实验中，具有极生鞭毛的霍乱弧菌与浓度不断增加的具有侧生鞭毛的副溶血性弧菌竞争，观察到具有侧生鞭毛的细菌抑制了具有极生鞭毛的细菌的吸附。相反，具有极生鞭毛的细菌增强了霍乱弧菌的吸附。在竞争实验中，副溶血性弧菌与浓度不断增加的其他细菌竞争，具有极生鞭毛的细菌显著增强了副溶血性弧菌的吸附，而具有侧生鞭毛的细菌仅略微增强了这一过程。观察到的表面饱和动力学、侧生鞭毛的产生以及具有侧生鞭毛的细菌抑制具有极生鞭毛的细菌吸附的能力之间的直接相关性表明，侧生鞭毛是细菌结构的一个组成部分，在具有侧生鞭毛的细菌吸附到表面的过程中很重要。基于所提供的证据，提出了一个具有侧生鞭毛的细菌吸附事件的模型。
