Donowitz M, Charney A N, Hynes R, Formal S B, Collins H
Infect Immun. 1979 Oct;26(1):380-6. doi: 10.1128/iai.26.1.380-386.1979.
In spite of several macroscopic criteria for predicting the presence of histological abnormalities in rabbit ileum, microscopic ileal abnormalities still can escape detection. The effect of histologically abnormal rabbit ileum was evaluated on basal intestinal absorption, on basal absorption, on basal adenylate cyclase activity, and on cholera toxin-induced secretion and cholera toxin-induced stimulation of adenylate cyclase activity. Compared to histologically normal rabbit ileum, the presence of histological abnormalities was associated with decreased basal intestinal water, Na, Cl, and glucose absorption, absent glucose-dependent water absorption, and elevated basal adenylate cyclase activities. However, histologically abnormal rabbit ileum responded to inoculation of purified cholera toxin with stimulation of intestinal water secretion and adenylate cyclase activity similar to that in histologically normal ileum. These data have implications concerning the design of experiments that attempt to study the pathogenesis of diarrheal diseases by correlating changes in ileal transport with changes in ileal mucosal adenylate cyclase activity. In spite of abnormal ileal histology, studies of intestinal secretory states which attempt to define the role of adenylate cyclase in secretory processes can be performed provided animals are used as their own controls. However, when groups of animals are compared, the presence of an histologically abnormal ileum can cause changes in basal and intestinal secretagogue-stimulated ileal water and electrolyte transport and in basal and intestinal secretagogue-stimulated mucosal adenylate cyclase activity which can lead to erroneous conclusions if the presence of the abnormal ileal histology is not considered.
尽管有几种宏观标准可用于预测兔回肠组织学异常的存在，但微观的回肠异常仍可能无法被检测到。本研究评估了组织学异常的兔回肠对基础肠吸收、基础吸收、基础腺苷酸环化酶活性、霍乱毒素诱导的分泌以及霍乱毒素诱导的腺苷酸环化酶活性刺激的影响。与组织学正常的兔回肠相比，组织学异常的存在与基础肠水、钠、氯和葡萄糖吸收减少、葡萄糖依赖性水吸收缺失以及基础腺苷酸环化酶活性升高有关。然而，组织学异常的兔回肠对接种纯化霍乱毒素的反应是肠水分泌和腺苷酸环化酶活性受到刺激，这与组织学正常的回肠相似。这些数据对于试图通过将回肠转运变化与回肠黏膜腺苷酸环化酶活性变化相关联来研究腹泻性疾病发病机制的实验设计具有启示意义。尽管回肠组织学异常，但只要将动物自身作为对照，就可以进行旨在确定腺苷酸环化酶在分泌过程中作用的肠分泌状态研究。然而，当比较不同组动物时，如果不考虑回肠组织学异常的存在，回肠组织学异常的存在可能会导致基础和肠促分泌剂刺激的回肠水和电解质转运以及基础和肠促分泌剂刺激的黏膜腺苷酸环化酶活性发生变化，从而得出错误的结论。
