Everitt E, Sundquist B, Philipson L
J Virol. 1971 Nov;8(5):742-53. doi: 10.1128/JVI.8.5.742-753.1971.
The mechanism of the arginine requirement for adenovirus was studied in cultures of KB cells infected with adenovirus type 2. Macromolecular synthesis was found to be severely impaired in uninfected cells under complete arginine deprivation, whereas an arginine concentration of 50 mum yielded a moderate and reversible inhibition of growth and nucleic acid synthesis. At this concentration, viral structural proteins were accumulated in excess although the virus yield was reduced more than 1,000-fold. The arginine-sensitive step appeared to occur early during the first 15 hr postinfection in the virus growth cycle. Virus-infected cells deprived of arginine to 50 mum showed, when reversed, a 4- to 5-hr lag period before the increase in virus growth was observed. Analysis of the radioactive pattern of labeled virions synthesized after reversion showed that all polypeptides were synthesized after addition of arginine to the medium, and none of the virion-polypeptides which are revealed by gel electrophoresis appeared to be preferentially synthesized after arginine reversion. The excess pool of structural proteins formed during depletion appeared to a large extent to be unavailable for virus assembly.
在感染2型腺病毒的KB细胞培养物中研究了腺病毒对精氨酸需求的机制。发现在完全缺乏精氨酸的情况下，未感染细胞中的大分子合成受到严重损害，而50μmol的精氨酸浓度会对生长和核酸合成产生中度且可逆的抑制。在此浓度下，尽管病毒产量降低了1000倍以上，但病毒结构蛋白仍会过量积累。精氨酸敏感步骤似乎发生在病毒生长周期感染后最初的15小时内。缺乏精氨酸至50μmol的病毒感染细胞在恢复精氨酸供应后，在观察到病毒生长增加之前有4至5小时的延迟期。对恢复供应后合成的标记病毒粒子的放射性模式分析表明，所有多肽都是在向培养基中添加精氨酸后合成的，凝胶电泳显示的病毒粒子多肽中，似乎没有一种在精氨酸恢复供应后被优先合成。在精氨酸缺乏期间形成的过量结构蛋白库在很大程度上似乎无法用于病毒组装。
