Philipson L, Lonberg-Holm K, Pettersson U
J Virol. 1968 Oct;2(10):1064-75. doi: 10.1128/JVI.2.10.1064-1075.1968.
HeLa or KB cells each contain around 10(4) receptor sites for adenovirus type 2. These are inactivated by treatment of live cells with subtilisin. The receptor activity of the enzyme-treated cells is regained after 4 to 8 hr of incubation in complete medium. A technique that utilizes the difference in buoyant density between free virus and virus-receptor complex was developed to demonstrate receptor activity. Cellular fractionation revealed that receptors were confined mainly to the plasma membrane fraction and that negligible receptor activity could be demonstrated in enzyme-treated cells. Subtilisin probably did not penetrate the cell membrane; thus, the receptors are limited to the cell surface. Purified fiber of the virion completely prevents attachment of adenovirus types 2 and 5 to receptor sites at a ratio of 10(5) protein molecules per cell. Adsorption studies indicate that 10(5) to 10(6) receptor sites are available for the structural protein. The fiber does not affect attachment of poliovirus type 1.
HeLa细胞或KB细胞各自含有约10⁴个2型腺病毒的受体位点。用枯草杆菌蛋白酶处理活细胞可使这些受体位点失活。在完全培养基中孵育4至8小时后，酶处理细胞的受体活性得以恢复。开发了一种利用游离病毒和病毒 - 受体复合物之间浮力密度差异的技术来证明受体活性。细胞分级分离显示受体主要局限于质膜部分，并且在酶处理的细胞中几乎检测不到受体活性。枯草杆菌蛋白酶可能没有穿透细胞膜；因此，受体仅限于细胞表面。病毒粒子的纯化纤维以每细胞10⁵个蛋白质分子的比例完全阻止2型和5型腺病毒与受体位点的附着。吸附研究表明有10⁵至10⁶个受体位点可用于结构蛋白。该纤维不影响1型脊髓灰质炎病毒的附着。
