Gill W B, Ruggiero K, Straus F H
Invest Urol. 1979 Nov;17(3):257-61.
We developed an in vivo technique to study crystallization in a urothelial-lined "living test tube" by employing the catheterized female rat bladder. Calcium oxalate crystals adherent to the bladder urothelium were distinguished from free or intraluminal crystals. Chemical injuries of the urothelium induced by either 0.1 N HCL or 5 per cent Triton X 100 in saline resulted in marked calcium oxalate crystal adhesion to the injured urothelium. Control bladders without urothelial injury remained free of adherent crystals but formed large numbers of small free crystals relatively uniform in size. Histologic and fresh microscopic evaluations of urothelial injury and crystal adhesion are presented.
我们开发了一种体内技术，通过使用经导管插入的雌性大鼠膀胱，在有尿路上皮衬里的“活体试管”中研究结晶现象。附着在膀胱尿路上皮的草酸钙晶体与游离或管腔内的晶体得以区分。用0.1N盐酸或盐水中5%的 Triton X 100对尿路上皮造成化学损伤，会导致草酸钙晶体明显附着在受损的尿路上皮上。未发生尿路上皮损伤的对照膀胱没有附着晶体，但形成了大量大小相对均匀的小游离晶体。本文展示了对尿路上皮损伤和晶体附着的组织学及新鲜显微镜评估。
