Gill W B, Ruggiero K J, Fromes M C
Invest Urol. 1980 Sep;18(2):158-61.
We studied the metastable limits of calcium oxalate supersaturation in glass tubes and in urothelial-lined rat bladders from a solvent system consisting of 0.9 per cent saline. The metastable limit was significantly elevated at 30 min in the urothelial-lined system as compared to the glass-surfaced containers. Heterogeneous nucleation on the container surfaces consistently occurred with the saline solvent in glass tubes as the metastable limit was exceeded. Intraluminal or free-floating crystals (? homogenous nucleation) without container-adherent crystals occurred with the saline solvent in urothelial-lined containers. This study reveals important properties of crystallization in urothelium and emphasizes the potential errors in extrapolating from crystallization studies in vitro in glass containers to crystallization phenomena in biological systems.
我们在玻璃管以及由0.9%生理盐水组成的溶剂系统灌注的大鼠膀胱上皮内衬中研究了草酸钙过饱和的亚稳极限。与玻璃表面容器相比，在膀胱上皮内衬系统中30分钟时亚稳极限显著升高。当超过玻璃管中生理盐水溶剂的亚稳极限时，容器表面持续发生异相成核。在膀胱上皮内衬容器中，生理盐水溶剂会产生腔内或自由漂浮的晶体（？均相成核），而没有附着在容器上的晶体。这项研究揭示了膀胱上皮中结晶的重要特性，并强调了从玻璃容器中的体外结晶研究推断生物系统中结晶现象时的潜在误差。
