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博拉腊病毒:从复杂瘤角蛾(鳞翅目:蝙蝠蛾科)中分离出的诺达病毒科成员。

Boolarra virus: a member of the Nodaviridae isolated from Oncopera intricoides (Lepidoptera: Hepialidae).

作者信息

Reinganum C, Bashiruddin J B, Cross G F

出版信息

Intervirology. 1985;24(1):10-7. doi: 10.1159/000149613.

DOI:10.1159/000149613
PMID:4044199
Abstract

A small RNA virus with a bipartite genome was isolated from Oncopera intricoides. It was named Boolarra virus (BoV). The particle had a diameter of 30 nm, a sedimentation coefficient of 140S, and a buoyant density of 1.34 g/ml in CsCl. The capsid proteins were examined by SDS-PAGE and consisted of a major species of molecular weight (mol. wt.) 38,000 and a minor one of mol. wt. 40,600. The particle contained 21.2% RNA which was divided between a 22S and a 17S species whose mol. wts. were 1.03 x 10(6) and 0.47 x 10(6), respectively. These properties were comparable to those of the Nodaviridae and established BoV as a member of the family. Serologically, BoV shared antigenic determinants with both Nodamura virus and black beetle virus.

摘要

从复杂澳夜蛾(Oncopera intricoides)中分离出一种具有双分体基因组的小RNA病毒。它被命名为博拉腊病毒(BoV)。该病毒粒子直径为30纳米,沉降系数为140S,在氯化铯中的浮力密度为1.34克/毫升。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)检测衣壳蛋白,其由一种主要的分子量为38,000的蛋白和一种次要的分子量为40,600的蛋白组成。该病毒粒子含有21.2%的RNA,分为22S和17S两种类型,其分子量分别为1.03×10⁶和0.47×10⁶。这些特性与诺达病毒科(Nodaviridae)的特性相当,从而确定BoV为该科的一个成员。在血清学上,BoV与诺达穆拉病毒(Nodamura virus)和黑甲虫病毒(black beetle virus)都有共同的抗原决定簇。

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