博拉腊病毒：从复杂瘤角蛾（鳞翅目：蝙蝠蛾科）中分离出的诺达病毒科成员。

Boolarra virus: a member of the Nodaviridae isolated from Oncopera intricoides (Lepidoptera: Hepialidae).

作者信息

Reinganum C, Bashiruddin J B, Cross G F

出版信息

Intervirology. 1985;24(1):10-7. doi: 10.1159/000149613.

Abstract

A small RNA virus with a bipartite genome was isolated from Oncopera intricoides. It was named Boolarra virus (BoV). The particle had a diameter of 30 nm, a sedimentation coefficient of 140S, and a buoyant density of 1.34 g/ml in CsCl. The capsid proteins were examined by SDS-PAGE and consisted of a major species of molecular weight (mol. wt.) 38,000 and a minor one of mol. wt. 40,600. The particle contained 21.2% RNA which was divided between a 22S and a 17S species whose mol. wts. were 1.03 x 10(6) and 0.47 x 10(6), respectively. These properties were comparable to those of the Nodaviridae and established BoV as a member of the family. Serologically, BoV shared antigenic determinants with both Nodamura virus and black beetle virus.

摘要

从复杂澳夜蛾（Oncopera intricoides）中分离出一种具有双分体基因组的小RNA病毒。它被命名为博拉腊病毒（BoV）。该病毒粒子直径为30纳米，沉降系数为140S，在氯化铯中的浮力密度为1.34克/毫升。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）检测衣壳蛋白，其由一种主要的分子量为38,000的蛋白和一种次要的分子量为40,600的蛋白组成。该病毒粒子含有21.2%的RNA，分为22S和17S两种类型，其分子量分别为1.03×10⁶和0.47×10⁶。这些特性与诺达病毒科（Nodaviridae）的特性相当，从而确定BoV为该科的一个成员。在血清学上，BoV与诺达穆拉病毒（Nodamura virus）和黑甲虫病毒（black beetle virus）都有共同的抗原决定簇。