Hajra A K, Burke C L, Jones C L
J Biol Chem. 1979 Nov 10;254(21):10896-900.
Upon differential centrifugation of rat liver homogenate, the enzyme acyl-CoA:dihydroxyacetone-phosphate acyltransferase (EC 2.3.1.42) was found to be localized in the light mitochondrial (L) fraction which is enriched with lysosomes and peroxisomes. Peroxisomes were separated from lysosomes in a density gradient centrifugation using rats which were injected with Triton WR 1339. By comparing the enzyme distribution with the distribution of different marker enzymes, it was concluded that dihydroxyacetone phosphate acyltransferase is primarily localized in rat liver peroxisomes (microbodies). Similarly, the enzyme acyl dihydroxyacetone-phosphate:NADPH oxidoreductase (EC 1.1.1.101) was shown to be enriched in the peroxisomal fraction, although a portion of this reductase is also present in the microsomal fraction.
对大鼠肝脏匀浆进行差速离心后发现，酰基辅酶A：磷酸二羟丙酮酰基转移酶（EC 2.3.1.42）定位于富含溶酶体和过氧化物酶体的轻线粒体（L）组分中。使用注射了 Triton WR 1339 的大鼠，通过密度梯度离心从溶酶体中分离出过氧化物酶体。通过比较该酶的分布与不同标记酶的分布，得出磷酸二羟丙酮酰基转移酶主要定位于大鼠肝脏过氧化物酶体（微体）中的结论。同样，酰基磷酸二羟丙酮：NADPH氧化还原酶（EC 1.1.1.101）也在过氧化物酶体组分中富集，尽管该还原酶的一部分也存在于微粒体组分中。
