Van Veldhoven P, Mannaerts G P
Biochem J. 1985 May 1;227(3):737-41. doi: 10.1042/bj2270737.
Peroxisomal (acyl-CoA oxidase and peroxisomal dihydroxyacetone-phosphate acyltransferase) and extraperoxisomal (mitochondrial fatty acid oxidation, extraperoxisomal dihydroxyacetone-phosphate acyltransferase, mitochondrial and microsomal glycerophosphate acyltransferases) lipid-metabolizing enzymes were measured in homogenates from rat liver and from seven extrahepatic tissues. Except for jejunal mucosa and kidney, extrahepatic tissues contained very little acyl-CoA oxidase activity. Peroxisomal dihydroxyacetone-phosphate acyltransferase, taken as the activity that was not inhibited by 5 mM-glycerol 3-phosphate, was present in all tissues examined, and its specific activity in liver and extrahepatic tissues was roughly of the same order of magnitude. Clofibrate treatment increased the activity of acyl-CoA oxidase in liver, and to a smaller extent also in kidney, but did not influence the activity of peroxisomal dihydroxyacetone-phosphate acyltransferase. Comparison of the activities of peroxisomal and extraperoxisomal lipid-metabolizing enzymes in extrahepatic tissues and in liver, an organ in which the contribution of peroxisomes to fatty acid oxidation and to glycerolipid synthesis has been estimated previously, suggests that, as in liver, peroxisomal long-chain fatty acid oxidation is of minor quantitative importance in extrahepatic tissues, but that in these tissues (micro)-peroxisomes are responsible for most of the dihydroxyacetone phosphate acylation and, consequently, for initiating ether glycerolipid synthesis.
在大鼠肝脏和七种肝外组织的匀浆中测定了过氧化物酶体（酰基辅酶A氧化酶和过氧化物酶体二羟基丙酮磷酸酰基转移酶）以及肝外（线粒体脂肪酸氧化、肝外二羟基丙酮磷酸酰基转移酶、线粒体和微粒体甘油磷酸酰基转移酶）脂质代谢酶。除空肠黏膜和肾脏外，肝外组织中酰基辅酶A氧化酶活性极低。以不受5 mM甘油3-磷酸抑制的活性作为过氧化物酶体二羟基丙酮磷酸酰基转移酶活性，在所检测的所有组织中均存在，其在肝脏和肝外组织中的比活性大致处于同一数量级。氯贝丁酯处理可增加肝脏中酰基辅酶A氧化酶的活性，在肾脏中也有较小程度的增加，但不影响过氧化物酶体二羟基丙酮磷酸酰基转移酶的活性。此前已估计过氧化物酶体对肝脏脂肪酸氧化和甘油olipid合成的贡献，比较肝外组织和肝脏中过氧化物酶体和肝外脂质代谢酶的活性表明，与肝脏一样，过氧化物酶体在肝外组织中对长链脂肪酸氧化的定量重要性较小，但在这些组织中，（微）过氧化物酶体负责大部分二羟基丙酮磷酸酰化，因此负责启动醚甘油olipid合成。 （注：原文中“glycerolipid”可能有误，推测应为“glycerolipid”，翻译为甘油脂质 ；“ether glycerolipid”翻译为醚甘油脂质 ）
