Jansen G A, Wanders R J
Department of Paediatrics and Clinical Biochemistry, University Hospital Amsterdam, The Netherlands.
J Inherit Metab Dis. 1997 Mar;20(1):85-94. doi: 10.1023/a:1005321910248.
Although ether-linked phospholipids have been known to be constituents of biological membranes for a long time, their physiological function has remained an enigma through the years. Inspired by the suggestion of Zoeller and coworkers that plasmalogens, which are specific types of ether-linked phospholipids characterized by the occurrence of an alpha, beta-unsaturated ether bond at the sn-1 position, are involved in the protection of cells against reactive oxygen species, we studied reactive oxygen species metabolism in cultured human skin fibroblasts. Menadione was used as intracellular generator of reactive oxygen species and cytochrome c as extracellular indicator for the production of reactive oxygen species. The finding that identical results were obtained in control and plasmalogen-deficient fibroblasts leads us to conclude that plasmalogens do not play a major role in protection against reactive oxygen species.
尽管醚键连接的磷脂作为生物膜的组成成分已为人所知许久，但多年来它们的生理功能一直是个谜。受佐勒及其同事的建议启发，即缩醛磷脂（一种特定类型的醚键连接的磷脂，其特征是在sn-1位存在α,β-不饱和醚键）参与保护细胞免受活性氧的侵害，我们研究了培养的人皮肤成纤维细胞中的活性氧代谢。用甲萘醌作为细胞内活性氧的生成剂，用细胞色素c作为细胞外活性氧产生的指示剂。在对照和成纤维细胞缺乏缩醛磷脂的细胞中获得相同结果这一发现，使我们得出结论，缩醛磷脂在抵御活性氧的保护作用中不发挥主要作用。
