Eberhard A
J Bacteriol. 1972 Mar;109(3):1101-5. doi: 10.1128/jb.109.3.1101-1105.1972.
Luciferase synthesis is repressed when bioluminescent bacteria are inoculated into fresh medium but is induced after the cells have grown in the medium for some time. In minimal medium, an activator which leads to induction of the enzyme is released into the medium by the bacteria. Complete medium contains a dialyzable and quite stable inhibitor which leads to repression of luciferase. The bacteria remove the inhibitor from the medium and also produce activator, thus allowing synthesis of the enzyme. Two unidentified nonluminescent strains of bacteria were unable to remove the inhibitor. Two different bioluminescent strains, Photobacterium fischeri and P. fischeri strain MAV, produce specific activators that are ineffective with cells of the other strain. The two activators are different with respect to heat stability, but both are small molecules. The activators can be assayed on the basis of their ability to counteract the inhibitor. Identification of the inhibitor and the activators may allow the bioluminescent system to be linked to other metabolic processes of the cells.
当将发光细菌接种到新鲜培养基中时，荧光素酶的合成受到抑制，但在细胞在培养基中生长一段时间后会被诱导。在基本培养基中，一种导致该酶诱导的激活剂由细菌释放到培养基中。完全培养基含有一种可透析且相当稳定的抑制剂，它会导致荧光素酶的抑制。细菌从培养基中去除抑制剂并产生激活剂，从而允许酶的合成。两种未鉴定的非发光细菌菌株无法去除抑制剂。两种不同的发光菌株，费氏弧菌和费氏弧菌MAV菌株，产生的特异性激活剂对另一种菌株的细胞无效。这两种激活剂在热稳定性方面不同，但都是小分子。可以根据它们对抗抑制剂的能力来测定激活剂。鉴定抑制剂和激活剂可能会使发光系统与细胞的其他代谢过程联系起来。
