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群体感应在水华形成中的作用:基因表达

The Role of Quorum Sensing in the Development of Blooms: Gene Expression.

作者信息

Lamas-Samanamud Gisella, Montante Armando, Mertins Andrea, Phan Duc, Loures Carla, Naves Fabiano, Reeves Tony, Shipley Heather J

机构信息

Department of Chemical and Materials Engineering, University of Kentucky, Paducah, KY 42002, USA.

School of Civil & Environmental Engineering, and Construction Management, University of San Antonio, San Antonio, TX 78249, USA.

出版信息

Microorganisms. 2023 Feb 2;11(2):383. doi: 10.3390/microorganisms11020383.

Abstract

is the dominant cyanobacterial species causing harmful algal blooms in water bodies worldwide. The blooms release potent toxins and pose severe public health hazards to water bodies, animals, and humans who are in contact with or consume this water. The interaction between and heterotrophic bacteria is thought to contribute to the development of the blooms. This study strives to provide a specific answer to whether quorum sensing is also a potential mechanism mediating the interaction of different strains/species and the expression by gene or gene in growth. The gene in PCC7806 is associated with quorum sensing and was tested by q-PCR throughout a 30-day growth period. The same was performed for the gene. Heterotrophic bacteria were collected from local water bodies: Cibolo Creek and Leon Creek in San Antonio, Texas. Results revealed that in algal bloom scenarios, there is a similar concentration of gene that is expressed by the cyanobacteria. Gene , however, is not directly associated with algal blooms, but it is related to cyanotoxin production. Toxicity levels increased in experiments with multiple algal strains, and the HSL treatment was not effective at reducing microcystin levels.

摘要

是导致全球水体有害藻华的主要蓝藻物种。藻华释放强效毒素,对水体、接触或饮用该水体的动物和人类构成严重的公共卫生危害。人们认为与异养细菌之间的相互作用有助于藻华的发展。本研究致力于具体回答群体感应是否也是介导不同菌株/物种相互作用以及基因或基因在生长过程中表达的潜在机制。集胞藻PCC7806中的基因与群体感应相关,并在30天的生长周期内通过定量聚合酶链反应进行检测。对基因也进行了同样的操作。异养细菌取自当地水体:得克萨斯州圣安东尼奥的锡博罗溪和莱昂溪。结果表明,在藻华情况下,蓝藻表达的基因浓度相似。然而,基因与藻华没有直接关联,但与蓝藻毒素的产生有关。在多个藻株的实验中,毒性水平有所增加,且高丝氨酸内酯处理在降低微囊藻毒素水平方面无效。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a49/9962132/503652faecd8/microorganisms-11-00383-g001.jpg

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