Higuchi M, Nishi K, Takenaka F
Jpn Heart J. 1979 Sep;20(5):667-73. doi: 10.1536/ihj.20.667.
Specific activities of enzymes in bovine hearts were measured. The enzyme activity ratios between the conduction system and the myocardium were 1.9 for G-6-PDH, 1.2 for PFK, 0.5 for total phosphorylase and LDH, 0.4 for GOT and MDH, 0.3 for SDH, 0.2 for Aldolase and CPK, and 0.1 for alpha GPDH. Approximate values for relative volume of Purkinje cells, nerve fibers and connective tissues in the conduction system were 30%, 8%, and 62%, respectively. It is concluded that activities of enzymes serving for anaerobic glycolysis in Purkinje cells are almost the same or slightly higher than those in the myocardium, and activity of enzyme for pentose shunt in the conductive tissue is higher than that in the myocardium.
测定了牛心脏中酶的比活性。传导系统与心肌之间的酶活性比值，葡萄糖-6-磷酸脱氢酶（G-6-PDH）为1.9，磷酸果糖激酶（PFK）为1.2，总磷酸化酶和乳酸脱氢酶（LDH）为0.5，谷草转氨酶（GOT）和苹果酸脱氢酶（MDH）为0.4，琥珀酸脱氢酶（SDH）为0.3，醛缩酶和肌酸磷酸激酶（CPK）为0.2，α-甘油磷酸脱氢酶（α GPDH）为0.1。传导系统中浦肯野细胞、神经纤维和结缔组织的相对体积近似值分别为30%、8%和62%。得出的结论是，浦肯野细胞中参与无氧糖酵解的酶的活性与心肌中的几乎相同或略高，而传导组织中戊糖旁路的酶活性高于心肌中的。
