Henry C G, Lowry O H
Am J Physiol. 1983 Nov;245(5 Pt 1):H824-9. doi: 10.1152/ajpheart.1983.245.5.H824.
Single canine Purkinje cells were isolated by microdissection and analyzed quantitatively for six enzymes of energy metabolism. Subendocardial Purkinje cells were clearly distinguishable morphologically and biochemically from adjacent myocardium and had enzyme levels comparable with extramural Purkinje cells. Oxidative enzymes, citrate synthase, malate dehydrogenase, and beta-hydroxyacyl CoA dehydrogenase were 40-60% lower in Purkinje cells than in myocardium. Lactate dehydrogenase was also 40% lower, but the other glycolytic enzymes, hexokinase and phosphofructokinase, were similar in level in myocardium and Purkinje cells. The results of this study show that it is completely practicable to apply quantitative histochemical analysis to biochemical study of the cardiac conducting system.
通过显微解剖分离出单个犬浦肯野细胞，并对六种能量代谢酶进行定量分析。心内膜下浦肯野细胞在形态和生化方面与相邻心肌明显不同，其酶水平与壁外浦肯野细胞相当。浦肯野细胞中的氧化酶、柠檬酸合酶、苹果酸脱氢酶和β-羟酰基辅酶A脱氢酶比心肌中的低40%-60%。乳酸脱氢酶也低40%，但其他糖酵解酶，己糖激酶和磷酸果糖激酶，在心肌和浦肯野细胞中的水平相似。本研究结果表明，将定量组织化学分析应用于心脏传导系统的生化研究是完全可行的。
