Frame M, Mollison P L
Immunology. 1972 Jun;22(6):1037-42.
The number of Hg sites on rabbit red cells was found to lie between 1.38 and 1.90 × 10 per cell in heterozygotes (HgHg) and between 3.0 and 3.8 × 10 per cell in homozygotes (HgHg). The lowest concentration of IgG anti-Hg which could be detected using a `standard' indirect antiglobulin test was in the range 0.02–0.08 μg/ml. The equilibrium constant of different examples of IgG anti-Hg lay between 0.5 and 1.56 × 101/mole. The ability of different examples of IgG anti-Hg to bind complement varied widely: some examples produced macroscopic haemolysis after 1-hour incubation with rabbit complement; one example produced no haemolysis even when the sensitive Cr-release method was used and failed to sensitize red cells to agglutination by anti-C3. The rate of haemolysis produced by haemolytic anti-Hg was very much less than that produced by haemolytic human anti-A.
在杂合子（HgHg）中，兔红细胞上汞位点的数量为每个细胞1.38至1.90×10个，在纯合子（HgHg）中为每个细胞3.0至3.8×10个。使用“标准”间接抗球蛋白试验能够检测到的最低IgG抗汞浓度在0.02 - 0.08μg/ml范围内。不同IgG抗汞样本的平衡常数在0.5至1.56×10¹/mol之间。不同IgG抗汞样本结合补体的能力差异很大：一些样本与兔补体孵育1小时后产生肉眼可见的溶血；一个样本即使采用敏感的铬释放法也不产生溶血，并且不能使红细胞对抗C3凝集敏感。溶血性抗汞产生的溶血速率远低于溶血性人抗A产生的溶血速率。
