Burton M S, Mollison P L
Immunology. 1968 Jun;14(6):861-78.
Production of the iso-antibody anti-Hg was stimulated in rabbits and relatively pure preparations of IgG and IgM anti-Hg were obtained by fractionating plasma on DEAE-cellulose. The effect of these preparations on red cell clearance was assessed by injecting different amounts, followed by Cr-labelled Hg-positive red cells, into previously untransfused Hg-negative rabbits. In rabbits injected with IgG antibody, the clearance of a small dose of red cells (0.01 ml/kg) always followed a single exponential curve even when red cell destruction was relatively slow, but in rabbits injected with small amounts of IgM antibody, two-component curves were observed, some cells being cleared very rapidly and others much more slowly. This difference between the effects of IgG and IgM antibodies had previously been suspected from work with human red cell iso-antibodies. Further experiments suggested that the destruction of only a proportion of transfused incompatible red cells, by low concentrations of IgM antibody, was due partly to `exhaustion' of antibody and partly to heterogeneity of the red cell population with respect to the antigen.
在兔子体内刺激产生了抗汞同种抗体，并通过在二乙氨基乙基纤维素上对血浆进行分级分离，获得了相对纯的抗汞IgG和IgM制剂。通过向先前未输血的汞阴性兔子注射不同剂量的制剂，随后注射铬标记的汞阳性红细胞，评估这些制剂对红细胞清除的影响。在注射IgG抗体的兔子中，即使红细胞破坏相对缓慢，小剂量红细胞（0.01 ml/kg）的清除始终遵循单指数曲线，但在注射少量IgM抗体的兔子中，观察到双组分曲线，一些细胞清除非常迅速，而另一些则慢得多。IgG和IgM抗体作用的这种差异先前已从人类红细胞同种抗体的研究中被怀疑。进一步的实验表明，低浓度的IgM抗体仅破坏一部分输入的不相容红细胞，部分原因是抗体“耗竭”，部分原因是红细胞群体在抗原方面的异质性。
