Harada F, Ikawa Y
Nucleic Acids Res. 1979 Oct 25;7(4):895-908. doi: 10.1093/nar/7.4.895.
A series of low molecular weight RNAs (4.5 to 5.5S) as well as other 4 to 7S RNAs were dissociated from genomic RNA of spleen focus forming virus (SFFV) by heating. On two dimensional polyacrylamide gel electrophoresis, this series of RNAs gave a series of more than thirty spots. RNase T1 fingerprints of these spots were identical except for differences in 3'-terminal oligonucleotides, which were mainly due to different numbers of uridylic acid residues, larger RNA-molecules containing poly(U)sequences at their 3'-termini. This series of RNAs is also associated with poly(A)-containing nuclear and cytoplasmic RNAs from SFFV-infected cells.
通过加热从脾脏集落形成病毒(SFFV)的基因组RNA中分离出一系列低分子量RNA(4.5至5.5S)以及其他4至7S RNA。在二维聚丙烯酰胺凝胶电泳上,这一系列RNA产生了一系列三十多个斑点。除了3'-末端寡核苷酸存在差异外,这些斑点的核糖核酸酶T1指纹图谱是相同的,这些差异主要是由于尿苷酸残基数量不同,较大的RNA分子在其3'-末端含有聚(U)序列。这一系列RNA也与来自SFFV感染细胞的含聚(A)的核RNA和细胞质RNA相关。