Landin R M, Boisnard M, Petrissant G
Nucleic Acids Res. 1979 Nov 24;7(6):1635-48. doi: 10.1093/nar/7.6.1635.
Histidyl-tRNAs from foetal and adult sheep liver were compared to their reticulocyte counterparts. The combination of various techniques revealed the existence of two histidyl-tRNA species in reticulocytes, one of which was not retained on acetylated DBAE-cellulose columns and was guanylatable. Three histidyl-tRNA isoacceptors were identified in foetal liver. Two of these species were not adsorbed on acetylated DBAE-cellulose but only one was found to be guanylatable. An identical chromatographic behaviour on RPC-5 columns was observed for guanylated histidyl-tRNAs from both origins. These results suggest the occurrence of a GUG anticodon in these guanine-accepting tRNAs. In foetal liver the amount of guanylatable histidyl-tRNA was estimated to be 7% of the total tRNA population. This observation is in agreement with the erythropoietic function of liver during the foetal life.
对来自胎儿和成年绵羊肝脏的组氨酰 - tRNA与其网织红细胞中的对应物进行了比较。多种技术的结合揭示了网织红细胞中存在两种组氨酰 - tRNA,其中一种不能保留在乙酰化二乙氨基乙基纤维素柱上且可被鸟苷化。在胎儿肝脏中鉴定出三种组氨酰 - tRNA同工受体。其中两种不能吸附在乙酰化二乙氨基乙基纤维素上,但只有一种被发现可被鸟苷化。来自两个来源的鸟苷化组氨酰 - tRNA在RPC - 5柱上表现出相同的色谱行为。这些结果表明这些鸟嘌呤接受性tRNA中存在GUG反密码子。在胎儿肝脏中,可被鸟苷化的组氨酰 - tRNA的量估计占总tRNA群体的7%。这一观察结果与胎儿期肝脏的红细胞生成功能一致。