兔网织红细胞无细胞提取物催化鸟嘌呤对tRNA反密码子中Q碱基的特异性取代。
Specific replacement of Q base in the anticodon of tRNA by guanine catalyzed by a cell-free extract of rabbit reticulocytes.
作者信息
Okada N, Harada F, Nishimura S
出版信息
Nucleic Acids Res. 1976 Oct;3(10):2593-603. doi: 10.1093/nar/3.10.2593.
Abstract
Guanylation of tRNA by a lysate of rabbit reticulocytes was reported previously by Farkas and Singh. This reaction was investigated further using 18 purified E. coli tRNAs as acceptors. Results showed that only tRNATyr, tRNAHis, tRNAAsn and tRNAAsp which contain the modified nucleoside Q in the anticodon acted as acceptors. Analysis of the nucleotide sequences in the guanylated tRNA showed that guanine specifically replaced Q base in these tRNAs.
摘要
法卡斯和辛格此前报道了兔网织红细胞裂解液对tRNA进行鸟苷酸化的情况。使用18种纯化的大肠杆菌tRNA作为受体对该反应进行了进一步研究。结果表明，只有反密码子中含有修饰核苷Q的tRNATyr、tRNAHis、tRNAAsn和tRNAAsp作为受体起作用。对鸟苷酸化tRNA的核苷酸序列分析表明，鸟嘌呤在这些tRNA中特异性取代了Q碱基。
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