Mizutani H
Jpn J Microbiol. 1975 Apr;19(2):157-62. doi: 10.1111/j.1348-0421.1975.tb00862.x.
Guinea pigs experimentally infected with Mycoplasma pneumoniae or immunized with the orgnaism in combination with Freund's complete adjuvant developed a delayed hypersensitive skin reaction following on intradermal injection of the M. pneumoniae antigen. The amount of protein necessary to produce the delayed skin reaction was as low as 0.01 mug. When the sonicated whole cells were extracted with aqueous acetone, the delayed skin reactivity was found mostly in the acetone insoluble (lipid-depleted) fraction. On the other hand, the lipid fraction which was isolated by a chloroform-methanol extraction of the acetone-soluble fraction and had a high titer of complement-fixing activity, exhibited little delayed skin reactivity. The lipid-depleted antigens as the whole cell antigens produced delayed skin reactivities in human patients.
用肺炎支原体实验感染豚鼠，或用该病原体与弗氏完全佐剂联合免疫豚鼠，在皮内注射肺炎支原体抗原后，这些豚鼠会出现迟发型超敏皮肤反应。产生迟发型皮肤反应所需的蛋白量低至0.01微克。当用丙酮水溶液提取超声破碎的全细胞时，发现迟发型皮肤反应性大多存在于丙酮不溶性（脱脂）部分。另一方面，通过对丙酮可溶性部分进行氯仿 - 甲醇提取分离得到的脂质部分，其补体固定活性效价高，但几乎没有迟发型皮肤反应性。脱脂抗原作为全细胞抗原在人类患者中产生了迟发型皮肤反应性。
