Korzeniowski O M, Barada F A, Rouse J D, Guerrant R L
Am J Trop Med Hyg. 1979 Nov;28(6):1031-5. doi: 10.4269/ajtmh.1979.28.1031.
Fecal specimens from 101 patients with diarrhea were cultured and also examined with methylene blue for leukocytes. Thirty-six patients had leukocytes in their stools and 29 had culture-proven shigellosis. The sensitivity of fecal leukocytes in shigellosis was 95% (19/20) when cup specimens were obtained, and 44% (4/9) when swab or diaper specimens were examined. Only 45% of the patients with shigellosis who provided cup specimens had grossly bloody dysentery. Twelve other patients had fecal leukocytes but no demonstrable invasive bacterial pathogens. Methylene blue examination was useful in identifying motile trophozoites of Giardia lamblia and eggs or larvae of other heavy intestinal paraistic infections. Among patients with naturally-acquired acute diarrhea, methylene blue examination of stools for leukocytes is much more sensitive than examination for blood in predicting a positive culture for Shigella spp. It is also of value in detecting parasites.
对101例腹泻患者的粪便标本进行培养，并使用亚甲蓝检测白细胞。36例患者粪便中有白细胞，29例经培养证实为志贺菌病。当采集杯状标本时，志贺菌病中粪便白细胞的敏感性为95%（19/20），而检查拭子或尿布标本时为44%（4/9）。提供杯状标本的志贺菌病患者中只有45%有明显的血性痢疾。另外12例患者粪便中有白细胞，但未发现可证实的侵袭性细菌病原体。亚甲蓝检查有助于识别蓝氏贾第鞭毛虫的活动滋养体以及其他重度肠道寄生虫感染的虫卵或幼虫。在自然获得性急性腹泻患者中，粪便亚甲蓝检查白细胞在预测志贺菌属阳性培养方面比检查血液更为敏感。它在检测寄生虫方面也有价值。
