通过反卷积法测定血浆蛋白合成率。
Rates of plasma protein synthesis by deconvolution.
作者信息
Norwich K H
出版信息
Biochem J. 1972 Mar;126(5):1124-6. doi: 10.1042/bj1261124.
The plasma specific radioactivity of arginine guanidine (14)C-labelled protein has been measured at various times after the injection of labelled protein precursor (Na(2) (14)CO(3)). The resulting ;appearance' curve is a balance between the rate of synthesis and delivery of new protein and the rate of destruction and distribution of new plus existing protein. The measured appearance curve can be corrected to give a measure solely of the rate of synthesis of the plasma protein concerned, if the amplitude of the curve at every point is increased by an amount that compensates for the destruction and distribution processes. The decrease of plasma specific radioactivity of labelled plasma protein after an injection of exogenously labelled protein is termed the ;disappearance' curve, and the method of correcting the appearance curve by using the disappearance curve is shown to take the form of a deconvolution.
在注射标记蛋白前体（Na₂¹⁴CO₃）后的不同时间，测量了精氨酸胍（¹⁴C标记）蛋白的血浆比放射性。所得的“出现”曲线是新蛋白合成与递送速率和新蛋白与现有蛋白的破坏与分布速率之间的平衡。如果曲线上每个点的幅度增加一个补偿破坏和分布过程的量，那么测量得到的“出现”曲线就可以校正，从而仅给出相关血浆蛋白的合成速率的度量。注射外源标记蛋白后，标记血浆蛋白的血浆比放射性降低，这被称为“消失”曲线，并且展示了通过使用“消失”曲线校正“出现”曲线的方法采取反卷积的形式。
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