氨酰基转移核糖核酸与大鼠肝脏核糖体结合过程中的中间反应。胆固醇14-甲基十六烷酸酯的相互作用。
Intermediate reactions in the binding of aminoacyl-transfer ribonucleic acid to rat liver ribosomes. the interaction of cholesteryl 14-methylhexadecanoate.
作者信息
Hradec J
出版信息
Biochem J. 1972 Mar;126(5):1225-9. doi: 10.1042/bj1261225.
Abstract
- Transferase I from rat liver extracted with iso-octane binds significantly less aminoacyl-tRNA than the non-extracted enzyme. The original activity can be fully restored by the addition of cholesteryl 14-methylhexadecanoate. The binding capacity for GTP is not affected by the extraction. 2. In the presence of extracted transferase I the binding of aminoacyl-tRNA to ribosomes is decreased to 11-26% and the simultaneous binding of GTP to 32-43%. Cholesteryl 14-methylhexadecanoate induces a full reactivation of the extracted enzyme in both respects. 3. Extracted complexes A (aminoacyl-tRNA-GTP-transferase I) become bound to ribosomes to the same extent as the corresponding non-extracted preparations. 4. It is concluded that cholesteryl 14-methylhexadecanoate interacts with the binding site of transferase I for aminoacyl-tRNA and secondarily with that for GTP. It does not affect the binding site for ribosomes.
摘要
- 用异辛烷提取的大鼠肝脏中的转移酶I与氨酰基-tRNA的结合量明显少于未提取的酶。通过添加14-甲基十六烷酸胆固醇酯可完全恢复其原始活性。对GTP的结合能力不受提取的影响。2. 在存在提取的转移酶I的情况下，氨酰基-tRNA与核糖体的结合减少到11%-26%，同时GTP的结合减少到32%-43%。14-甲基十六烷酸胆固醇酯在这两个方面都能使提取的酶完全重新激活。3. 提取的复合物A（氨酰基-tRNA-GTP-转移酶I）与核糖体的结合程度与相应的未提取制剂相同。4. 得出的结论是，14-甲基十六烷酸胆固醇酯与转移酶I的氨酰基-tRNA结合位点相互作用，其次与GTP结合位点相互作用。它不影响核糖体的结合位点。
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