用胆固醇酯酶处理后肽链延伸因子的活性降低。
Decreased activity of peptide-elongation factors after treatment with cholesterol esterase.
作者信息
Hradec J, Tuhácková Z, Dusek Z
出版信息
Biochem J. 1978 Apr 15;172(1):9-13. doi: 10.1042/bj1720009.
Abstract
- Peptide-elongation factors were purified from rat liver and treated with cholesterol esterase and phospholipase A2 immobilized on Sepharose 4B. 2. Binding of L-[3H]-phenylalanyl-tRNA to 40S ribosomal subunits was decreased by approx. 70% and to polyribosomes by 30% in the presence of the binding factor incubated with cholesterol esterase. Treatment of this factor with immobilized phospholipase A2 decreased the binding to smaller ribosomal subunits by only about 15%. 3. Poly(U)-dependent phenylalanine polymerization by ribosomal subunits was decreased to approx. 30% of its original value by treatment of both elongation factors with cholesterol esterase. 4. The normal activity of esterase-treated elongation factor in both the binding reaction and peptide-elongation assay was fully recovered by the addition of cholesteryl 14-methyl-hexadecanoate. 5. Different classes of lipids present in peptide-elongation factor 1 have apparently different functions. Whereas phospholipids are required to maintain the strcture of heavy aggregates of this factor, the presence of cholesteryl 14-methylhexadecanoate is obviously necessary for the normal function of peptide-elongation factors.
摘要
- 从大鼠肝脏中纯化出肽链延长因子，并用固定在琼脂糖4B上的胆固醇酯酶和磷脂酶A2进行处理。2. 在与胆固醇酯酶孵育的结合因子存在的情况下，L-[3H]-苯丙氨酰-tRNA与40S核糖体亚基的结合减少了约70%，与多核糖体的结合减少了30%。用固定化磷脂酶A2处理该因子，与较小核糖体亚基的结合仅减少约15%。3. 通过用胆固醇酯酶处理两种延长因子，核糖体亚基的多聚尿苷酸依赖性苯丙氨酸聚合反应降至其原始值的约30%。4. 通过添加14-甲基十六烷酸胆固醇酯，酯酶处理的延长因子在结合反应和肽链延长测定中的正常活性得以完全恢复。5. 肽链延长因子1中存在的不同类脂质显然具有不同功能。虽然磷脂是维持该因子重聚集体结构所必需的，但14-甲基十六烷酸胆固醇酯的存在显然是肽链延长因子正常功能所必需的。
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Decreased activity of peptide-elongation factors after treatment with cholesterol esterase.用胆固醇酯酶处理后肽链延伸因子的活性降低。
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引用本文的文献
1
Cholesteryl esters are bound by a peptide-initiation and a peptide-elongation factor.胆固醇酯由一种肽起始因子和一种肽延伸因子结合。
Biochem J. 1980 Oct 15;192(1):369-72. doi: 10.1042/bj1920369.
2
Heparin-sepharose 4B at low temperatures retains ribosomes.低温下的肝素琼脂糖4B能保留核糖体。
Biochem J. 1978 Jul 1;173(1):349-52. doi: 10.1042/bj1730349.
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Purification and some properties of a protein factor binding and deacylating initiator transfer ribonucleic acid.一种与起始转运核糖核酸结合并使其脱酰基的蛋白质因子的纯化及某些特性
Biochem J. 1979 Feb 1;177(2):707-19. doi: 10.1042/bj1770707.
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The role of cholesteryl 14-methylhexadecanoate in peptide elongation reactions.胆固醇14-甲基十六烷酸酯在肽链延伸反应中的作用。
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