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地衣芽孢杆菌中壁磷壁酸的生物合成。

Biosynthesis of the wall teichoic acid in Bacillus licheniformis.

作者信息

Hancock I C, Baddiley J

出版信息

Biochem J. 1972 Mar;127(1):27-37. doi: 10.1042/bj1270027.

Abstract
  1. The biosynthesis of the wall teichoic acid, poly(glycerol phosphate glucose), has been studied with a particulate membrane preparation from Bacillus licheniformis A.T.C.C. 9945. The precursor CDP-glycerol supplies glycerol phosphate residues, whereas UDP-glucose supplies only glucose to the repeating structure of the polymer. 2. Synthesis proceeds through polyprenol phosphate derivatives, and chemical studies and pulse-labelling techniques show that the first intermediate is the phosphodiester, glucose polyprenol monophosphate. CDP-glycerol donates a glycerol phosphate residue to this to give a second intermediate, (glycerol phosphate glucose phosphate) polyprenol. 3. The glucose residue in the lipid intermediates has the beta configuration, and chain extension in the synthesis of polymer occurs by transglycosylation with inversion of anomeric configuration at two stages.
摘要
  1. 利用地衣芽孢杆菌A.T.C.C. 9945的颗粒状膜制剂,对壁磷壁酸(聚甘油磷酸葡萄糖)的生物合成进行了研究。前体CDP - 甘油提供甘油磷酸残基,而UDP - 葡萄糖仅为聚合物的重复结构提供葡萄糖。2. 合成通过聚戊烯醇磷酸衍生物进行,化学研究和脉冲标记技术表明,第一个中间体是磷酸二酯,葡萄糖聚戊烯醇单磷酸。CDP - 甘油将一个甘油磷酸残基捐赠给它,从而产生第二个中间体,(甘油磷酸葡萄糖磷酸)聚戊烯醇。3. 脂质中间体中的葡萄糖残基具有β构型,聚合物合成中的链延伸通过转糖基化发生,在两个阶段异头构型发生反转。

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An acceptor-dependent polyglycerolphosphate polymerase.一种依赖受体的聚甘油磷酸聚合酶。
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Immunological properties of teichoic acids.磷壁酸的免疫学特性。
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本文引用的文献

1
Separation of the phosphoric esters on the filter paper chromatogram.磷酸酯在滤纸色谱上的分离
Nature. 1949 Dec 31;164(4183):1107-12, illust. doi: 10.1038/1641107a0.
2
Detection of sugars on paper chromatograms.纸色谱上糖的检测
Nature. 1950 Sep 9;166(4219):444-5. doi: 10.1038/166444b0.
4
Studies on uridine-diphosphate-glucose.尿苷二磷酸葡萄糖的研究。
Biochem J. 1952 Jun;51(3):426-30. doi: 10.1042/bj0510426.

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