用于测定叶蛋白浓缩物中活性赖氨酸的染料结合法和氟二硝基苯法的比较。

A comparison of the dye-binding and fluorodinitrobenzene methods for determining reactive lysine in leaf-protein concentrates.

Walker A F

Br J Nutr. 1979 Nov;42(3):455-65. doi: 10.1079/bjn19790137.

Abstract

Twenty-eight leaf-protein concentrate (LPC) samples, subjected to different thermal treatments, were produced from five curd batches. For these samples, the fluorodinitrobenzene (FDNB)-reactive lysine values gave closer agreement with dye-binding lysine (DBL) than with the dye-binding capacity (DBC). 2. No relationship was established between the dye-bound-after-propionylation (DBAP) and the histidine + arginine value. 3. Comparison of dye-bound-protein values with those for tungstic-acid-precipitated nitrogen x 6.25 for the LPC samples showed the heat-damaged samples to lie below the regression line for the other samples. 4. Reactive-lysine values by dye-binding and by FDNB methods correlated well with total lysine, but the slopes of the regression line indicated closer agreement for values for samples not damaged by heat. 5. The correlation coefficients between DBC and total basic amino acids, DBC and histidine + arginine + DBL, and DBC and histidine + arginine + FDNB-reactive lysine were similar. 6. There was no correlation between the lightness of colour of the LPC samples and the availability of lysine.

摘要

从五批凝乳中制备了28个经过不同热处理的叶蛋白浓缩物（LPC）样品。对于这些样品，氟二硝基苯（FDNB）反应性赖氨酸值与染料结合赖氨酸（DBL）的一致性比与染料结合能力（DBC）的一致性更高。2. 丙酰化后染料结合量（DBAP）与组氨酸+精氨酸值之间未建立关系。3. 将LPC样品的染料结合蛋白值与钨酸沉淀氮×6.25的值进行比较，结果表明热损伤样品位于其他样品的回归线以下。4. 通过染料结合法和FDNB法测得的反应性赖氨酸值与总赖氨酸相关性良好，但回归线的斜率表明未受热损伤的样品的值一致性更高。5. DBC与总碱性氨基酸、DBC与组氨酸+精氨酸+DBL以及DBC与组氨酸+精氨酸+FDNB反应性赖氨酸之间的相关系数相似。6. LPC样品的颜色亮度与赖氨酸的可利用性之间没有相关性。