Haski R, Stewart B W
Cancer Lett. 1979 Sep;7(5):283-8. doi: 10.1016/s0304-3835(79)80055-5.
Structural analysis of rat lung DNA was made by stepwise elution of the nucleic acid from benzoylated--DEAE-cellulose with 1.0 M NaCl and 30% formamide solutions respectively, the DNA content of collected fractions being determined by absorbance. The proportion of the minor fraction, which was eluted with formamide and thought to contain DNA having single-stranded regions, was increased by intratracheal instillation of 3-methylcholanthrene prior to death of the animal. Maximum initial increase was observed 14 h after treatment. Analysed up to 10 days after treatment, there was a biphasic response, the early maxima being dose dependent and the second apparently independent of the amount of hydrocarbon administered. The data implicate DNA repair processes and cellular proliferation stimulated by 3-methylcholanthrene as being involved in recovery of the lung from this potentially carcinogenic insult.
通过分别用1.0M氯化钠溶液和30%甲酰胺溶液从苯甲酰化的二乙氨基乙基纤维素上逐步洗脱核酸，对大鼠肺DNA进行结构分析，收集的各部分的DNA含量通过吸光度来测定。用甲酰胺洗脱的、被认为含有具有单链区域的DNA的次要部分的比例，在动物死亡前经气管内滴注3-甲基胆蒽后增加。治疗后14小时观察到最大的初始增加。在治疗后长达10天的分析中，出现了双相反应，早期的最大值呈剂量依赖性，而第二个最大值显然与所给予的碳氢化合物的量无关。这些数据表明，3-甲基胆蒽刺激的DNA修复过程和细胞增殖参与了肺从这种潜在致癌损伤中的恢复。
