Bednarík T, Simek L, Cajthamlová H, Rerábková E, Jezková Z, Kout M
Folia Haematol Int Mag Klin Morphol Blutforsch. 1975;102(1):48-55.
The possibility has been investigated of a direct gain of ALS free of undesirable antibodies against serum proteins by inducing immunologic tolerance in productive animals (pigs). Preliminary experiments made with tolerogenic amounts of 10 and 50 ml of sera and with immunization by the serum alone proved applicability of this method. Electrophoresis showed antibodies against 6 to 7 and 2 to 3 fractions in animals tolerated with 10 and 50 ml respectively, compared to 18 to 20 fractions in the control group, which was not tolerated. This has been confirmed when preparing ALS in practice, where the toleration was carried out with 25 ml of serum or with the same amount of serum with the addition of hemoglobin and immunization by lymphocytes isolated from peripheral blood. Final ALS of untolerated animals contained antibodies against 7 to 8 fractions, whereas that of experimental group tolerated with serum and Hb was free of antibodies against serum protein, hemoglobin included. ALS of the group tolerated with normal serum contained only antibodies against hemoglobin. In vitro tests (i.e. lymphoagglutination t., lymphocytotoxicity t., rosette inhibition t.) proved that by inducing tolerance towards serum protein the activity of ALS was in no was affected. According to the results this method can be employed not only for the preparation of ALS, but also for other purposes, such as preparation of monovalent antisera for immunoelectrophoresis.
通过诱导生产性动物（猪）产生免疫耐受，研究了直接获得不含针对血清蛋白的不良抗体的抗淋巴细胞血清（ALS）的可能性。用10毫升和50毫升致耐受性血清量以及仅用血清进行免疫的初步实验证明了该方法的适用性。电泳显示，分别用10毫升和50毫升血清耐受的动物中，针对6至7个和2至3个组分存在抗体，而未耐受的对照组中有18至20个组分。在实际制备ALS时得到了证实，即用25毫升血清或等量添加血红蛋白的血清进行耐受，并通过从外周血中分离的淋巴细胞进行免疫。未耐受动物的最终ALS含有针对7至8个组分的抗体，而用血清和血红蛋白耐受的实验组的ALS不含针对血清蛋白（包括血红蛋白）的抗体。用正常血清耐受的组的ALS仅含有针对血红蛋白的抗体。体外试验（即淋巴细胞凝集试验、淋巴细胞毒性试验、玫瑰花结抑制试验）证明，通过诱导对血清蛋白的耐受性，ALS的活性丝毫未受影响。根据结果，该方法不仅可用于制备ALS，还可用于其他目的，如制备用于免疫电泳的单价抗血清。
