Bednarík T, Simek L, Cajthamlová H, Rerábková E, Jezková Z, Májský A, Kout M
Folia Haematol Int Mag Klin Morphol Blutforsch. 1976;103(1):76-83.
Lymphocytes of donors' peripheral blood were used for the study of pig antihuman ALS preparation by means of local and intravenous immunization. The results were compared with those obtained with exclusively local application of the same antigen. Antisera were tested from the aspect of activity (lymphoagglutination, lymphocytotoxicity, the rosette inhibition test) and for the presence of unwanted antibodies (haemagglutinins, thromboagglutinins, precipitins to serum proteins). Of all alternatives tested the following scheme proved to be optimal: subcutaneous application of 2 X 10(9) lymphocytes with adjuvans on day 0, followed by intravenous application of the same dosage on day 13, and serum harvesting on day 20. Antisera thus obtained displayed titres 1: 32 000 to 1 : 130 000 in the RIT - this being maximum level reached by exclusively local immunization only in some animals. Therefore, favourable immunosuppressive effect in vivo can be expected. Even more profound difference was observed in the level of unwanted antibodies that were found to be on markedly lower level after combined immunization. There is a good reason to suppose that with the help of further techniques, more likely absorption, these can be lowered to acceptable level. It is apparent from the results that with the help of a suitable immunization procedure not only highly active ALS can be obtained but also formation of nondesirable antibodies can be suppressed and, despite their thrombocyte contamination peripheral blood lymphocytes can successfully be used.
采用供体外周血淋巴细胞，通过局部和静脉免疫的方法来研究猪抗人抗淋巴细胞血清（ALS）制剂。将结果与仅采用相同抗原局部应用所获得的结果进行比较。从活性方面（淋巴细胞凝集、淋巴细胞毒性、玫瑰花结抑制试验）以及是否存在不需要的抗体（血凝素、血栓凝集素、血清蛋白沉淀素）对抗血清进行检测。在所有测试的方案中，以下方案被证明是最佳的：在第0天皮下注射2×10⁹个淋巴细胞加佐剂，然后在第13天静脉注射相同剂量，在第20天采集血清。如此获得的抗血清在玫瑰花结抑制试验（RIT）中的效价为1:32000至1:130000，而仅局部免疫时只有部分动物能达到这一最高水平。因此，可以预期在体内会有良好的免疫抑制效果。在不需要的抗体水平上观察到了更显著的差异，联合免疫后发现这些抗体水平明显更低。有充分的理由认为，借助进一步的技术，更有可能是吸收技术，这些抗体水平可以降低到可接受的水平。从结果可以明显看出，借助合适的免疫程序，不仅可以获得高活性的ALS，而且可以抑制不需要的抗体的形成，并且尽管外周血淋巴细胞存在血小板污染，但仍可成功使用。
