Shibaev V N, Druzhinina T N, Popova A N, Rozhnova S S, Kilesso V A
Eur J Biochem. 1979 Nov 1;101(1):309-16. doi: 10.1111/j.1432-1033.1979.tb04244.x.
Cell envelope and soluble glycosyl transferase preparations from Salmonella newport (serogroup C2) and Salmonella kentucky (serogroup C3) were found to catalyze formation of polyprenyl pyrophosphate tetrasaccharides corresponding to the structure of the repeating unit of the main chain of O-specific polysaccharides. Plant polyprenyl phosphate may serve as an exogenous sugar acceptor. Galactose residue is an initiator of a chain growth: transfer of galactosyl phosphate from uridine diphosphate galactose onto the acceptor is followed by two consecutive mannosyl transfers from guanosine diphosphate mannose and rhamnosyl transfer thymidine diphosphate rhamnose. Uridine diphosphate glucose and polyprenyl phosphate are converted by the enzyme preparations into polyprenyl monophosphate glucose which may transfer a glucosyl residue onto the polyprenyl pyrophosphate oligosaccharides. The resulting pentasaccharide derivatives may be polymerised by enzymes present in cell envelope preparations. The significance of these results for the understanding of the mechanism of O-specific polysaccharide biosynthesis is discussed.
已发现来自新港沙门氏菌（血清群C2）和肯塔基沙门氏菌（血清群C3）的细胞包膜和可溶性糖基转移酶制剂可催化形成与O-特异性多糖主链重复单元结构相对应的聚异戊二烯焦磷酸四糖。植物聚异戊二烯磷酸可作为外源糖受体。半乳糖残基是链生长的起始物：磷酸半乳糖从尿苷二磷酸半乳糖转移到受体上，随后是来自鸟苷二磷酸甘露糖的两次连续甘露糖基转移和来自胸苷二磷酸鼠李糖的鼠李糖基转移。尿苷二磷酸葡萄糖和聚异戊二烯磷酸被酶制剂转化为聚异戊二烯单磷酸葡萄糖，其可将葡萄糖基残基转移到聚异戊二烯焦磷酸寡糖上。所得的五糖衍生物可通过细胞包膜制剂中存在的酶进行聚合。讨论了这些结果对于理解O-特异性多糖生物合成机制的意义。
