Gielen H G, Wolf G, Berndt H, Zahn H
Hoppe Seylers Z Physiol Chem. 1979 Nov;360(11):1535-48.
The following paper describes the synthesis of the fragments A1-8, A9-15 and A16-21 of the ovine insulin A chain using the S-tert-butylmercapto residue for thiol protection. The synthesized fragments, which showed a good solubility in organic solvents, were partially deprotected with trifluoracetic acid and converted to the corresponding S-sulfonates quantitatively by oxidative sulfitolyses at pH 7.6.
以下论文描述了使用S-叔丁基巯基残基进行硫醇保护来合成绵羊胰岛素A链的A1-8、A9-15和A16-21片段。合成的片段在有机溶剂中具有良好的溶解性，用三氟乙酸进行部分脱保护，并在pH 7.6下通过氧化亚硫酸解法定量转化为相应的S-磺酸盐。
