C1和C2化合物的微生物代谢。乙醛酸、乙醇酸和乙酸盐在假单胞菌AM1利用乙醇和C1化合物生长过程中的作用。
Microbial metabolism of C 1 and C 2 compounds. The role of glyoxylate, glycollate and acetate in the growth of Pseudomonas AM1 on ethanol and on C 1 compounds.
作者信息
Dunstan P M, Anthony C, Drabble W T
出版信息
Biochem J. 1972 Jun;128(1):107-15. doi: 10.1042/bj1280107.
Abstract
Succinate (or a product of succinate metabolism) is a catabolite repressor of some enzymes of the serine pathway (hydroxypyruvate reductase, serine-glyoxylate aminotransferase and glycerate kinase) but not of methanol dehydrogenase nor methylamine dehydrogenase. A mutant (PCT64) of Pseudomonas AM1, which is unable to grow on C(1) compounds, lacks glycerate kinase, showing that this enzyme is essential for the operation of the serine pathway. Mutant PCT48, unable to convert acetate into glycollate, has lost the ability to grow both on C(1) compounds and on ethanol. The properties of a third mutant (PCT57) show that Pseudomonas AM1 contains enzymes catalysing the conversion of acetate into glyoxylate. Evidence is presented that hydroxypyruvate reductase is involved in the oxidation of glycollate to glyoxylate during growth on ethanol. A scheme is proposed for the conversion of ethanol and of C(1) compounds into glyoxylate in which acetate (or a derivative) and glycollate are intermediates.
摘要
琥珀酸（或琥珀酸代谢产物）是丝氨酸途径某些酶（羟基丙酮酸还原酶、丝氨酸-乙醛酸转氨酶和甘油酸激酶）的分解代谢阻遏物，但不是甲醇脱氢酶和甲胺脱氢酶的分解代谢阻遏物。不能在C(1)化合物上生长的假单胞菌AM1突变体（PCT64）缺乏甘油酸激酶，这表明该酶对丝氨酸途径的运行至关重要。无法将乙酸盐转化为乙醇酸的突变体PCT48失去了在C(1)化合物和乙醇上生长的能力。第三个突变体（PCT57）的特性表明，假单胞菌AM1含有催化乙酸盐转化为乙醛酸的酶。有证据表明，羟基丙酮酸还原酶在乙醇生长过程中参与乙醇酸氧化为乙醛酸的过程。提出了一个将乙醇和C(1)化合物转化为乙醛酸的方案，其中乙酸盐（或衍生物）和乙醇酸是中间体。
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本文引用的文献
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Microbial growth on C(1) compounds. 5. Enzyme activities in extracts of Pseudomonas AM1.C(1) 化合物上的微生物生长。5. 假单胞菌AM1提取物中的酶活性。
Biochem J. 1963 May;87(2):386-96. doi: 10.1042/bj0870386.
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Microbial growth on C1 compounds. II. Synthesis of cell constituents by methanol- and formate-grown Pseudomonas AM 1, and methanol-grown Hyphomicrobium vulgare.C1化合物上的微生物生长。II. 甲醇和甲酸培养的假单胞菌AM 1以及甲醇培养的普通生丝微菌的细胞成分合成
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The metabolism of C2 compounds in micro-organisms. 6. Synthesis of cell constituents from glycollate by Pseudomonas sp.微生物中C2化合物的代谢。6. 假单胞菌属细菌从乙醇酸盐合成细胞成分
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Microbial growth on oxalate by a route not involving glyoxylate carboligase.微生物通过不涉及乙醛酸羧化酶的途径在草酸盐上生长。
Biochem J. 1970 Jun;118(1):53-9. doi: 10.1042/bj1180053.
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Aspects of glycine and serine biosynthesis during growth of Pseudomonas AM1 on C compounds.在假单胞菌AM1利用碳源化合物生长过程中甘氨酸和丝氨酸生物合成的相关方面。
Biochem J. 1971 Mar;121(5):763-9. doi: 10.1042/bj1210763.
7
Microbial metabolism of C 1 and C 2 compounds. The involvement of glycollate in the metabolism of ethanol and of acetate by Pseudomonas AM1.C1和C2化合物的微生物代谢。乙醇酸在假单胞菌AM1对乙醇和乙酸盐代谢中的作用。
Biochem J. 1972 Jun;128(1):99-106. doi: 10.1042/bj1280099.
8
Purification and properties of an amine dehydrogenase from Pseudomonas AM1 and its role in growth on methylamine.来自假单胞菌AM1的胺脱氢酶的纯化、性质及其在甲胺生长中的作用。
Biochem J. 1968 Jan;106(1):245-55. doi: 10.1042/bj1060245.
9
The biosynthesis of serine and glycine in Pseudomonas AM1 with special reference to growth on carbon sources other than C1 compounds.假单胞菌AM1中丝氨酸和甘氨酸的生物合成，特别涉及以除C1化合物以外的碳源生长的情况。
Biochem J. 1971 Mar;121(5):753-62. doi: 10.1042/bj1210753.
10
Pathways leading to and from serine during growth of Pseudomonas AM1 on C1 compounds or succinate.在假单胞菌AM1利用C1化合物或琥珀酸盐生长期间，丝氨酸的合成与代谢途径。
Biochem J. 1970 Apr;117(3):563-72. doi: 10.1042/bj1170563.
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