哺乳动物细胞核中的脱氧核糖核酸合成。脱氧核糖核苷酸和链终止核苷酸类似物的掺入。
Deoxyribonucleic acid synthesis in mammalian nuclei. Incorporation of deoxyribonucleotides and chain-terminating nucleotide analogues.
作者信息
Waqar M A, Burgoyne L A, Atkinson M R
出版信息
Biochem J. 1971 Mar;121(5):803-9. doi: 10.1042/bj1210803.
Abstract
The properties of a nuclear preparation from rat liver and thymus are described. (1) Nearest-neighbour analysis after incorporation of (32)P-labelled nucleotide residues from dATP, dCTP, dGTP, dTTP and arabinofuranosyl analogues of CTP and ATP shows template-dependent DNA synthesis. (2) Where primer termini are limiting, incorporation of arabinofuranosyl analogues of AMP and CMP residues proceeds to a limit indicating that both of these analogues are DNA chain terminators. (3) No large differences have been found between the priming potentialities or the intrinsic DNA polymerase activities of nuclei from resting or regenerating liver and the relationship of this DNA synthesis in vitro to DNA replication or repair in vivo is briefly discussed.
摘要
本文描述了大鼠肝脏和胸腺的核制剂的特性。(1) 掺入来自dATP、dCTP、dGTP、dTTP以及CTP和ATP的阿拉伯呋喃糖基类似物的(32)P标记核苷酸残基后的最近邻分析表明存在模板依赖性DNA合成。(2) 在引物末端有限的情况下，AMP和CMP残基的阿拉伯呋喃糖基类似物的掺入达到极限，表明这两种类似物都是DNA链终止剂。(3) 在静止或再生肝脏的核的引发潜力或内在DNA聚合酶活性之间未发现大的差异，并简要讨论了这种体外DNA合成与体内DNA复制或修复的关系。
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本文引用的文献
1
Studies on the incorporation of deoxyribonucleic acid.关于脱氧核糖核酸掺入的研究。
Biochim Biophys Acta. 1962 May 14;55:639-50. doi: 10.1016/0006-3002(62)90842-9.
2
POLYDEOXYADENYLIC ACID.聚脱氧腺苷酸
Proc Natl Acad Sci U S A. 1964 May;51(5):853-9. doi: 10.1073/pnas.51.5.853.
3
4
5
Nuclei from rat liver: isolation method that combines purity with high yield.大鼠肝脏细胞核：一种将纯度与高产率相结合的分离方法。
Science. 1966 Dec 30;154(3757):1662-5. doi: 10.1126/science.154.3757.1662.
6
Preparation of [alpha-32P]nucleoside and deoxynucleoside 5'-triphosphates from 32Pi and protected and unprotected nucleosides.由³²Pi以及受保护和未受保护的核苷制备[α-³²P]核苷和脱氧核苷5'-三磷酸。
Biochim Biophys Acta. 1969 Oct 22;190(2):548-50. doi: 10.1016/0005-2787(69)90105-1.
7
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Biochemistry. 1969 Dec;8(12):4897-904. doi: 10.1021/bi00840a037.
8
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9
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