Hewitt H B, Blake E R
Br J Cancer. 1971 Jun;25(2):323-36. doi: 10.1038/bjc.1971.42.
Within 48 hours of the institution of severe phenylhydrazineinduced anaemia in mice bearing ascites tumours or generalised leukaemia, a substantial proportion of the malignant cells disappeared respectively from the peritoneal cavity or infiltrated liver. The results of radiobiological experiments permitting determination of the proportion of viable leukaemia cells which were severely hypoxic and relatively radioresistant in the livers of leukaemic mice, showed that induction of anaemia was associated with a several hundredfold increase in the proportion of such cells. The proportion of hypoxic cells was greatly reduced when the anaemic leukaemic mice were transfused with packed erythrocytes or allowed to breathe oxygen under high pressure. Similar experi - ments with solid sarcomas indicated that a high proportion of the tumour cells were hypoxic in non-anaemic mice breathing air. The hypoxic fraction was not significantly reduced when tumour-bearing mice were made severely anaemic during growth of the tumour and were later transfused. Thus, the hypoxic cells in leukaemic livers and those in solid tumours are markedly different in their capacity for oxygenation following the induction of relative hyperoxaemia.
在患有腹水肿瘤或全身性白血病的小鼠中，用苯肼诱发严重贫血后48小时内，相当一部分恶性细胞分别从腹腔消失或从浸润的肝脏中消失。放射生物学实验结果能够确定白血病小鼠肝脏中严重缺氧且相对抗辐射的存活白血病细胞的比例，结果显示贫血的诱导与这类细胞比例增加数百倍有关。当给贫血的白血病小鼠输注浓缩红细胞或使其在高压下吸氧时，缺氧细胞的比例会大大降低。对实体肉瘤进行的类似实验表明，在呼吸空气的非贫血小鼠中，很大一部分肿瘤细胞是缺氧的。在肿瘤生长期间使荷瘤小鼠严重贫血，随后进行输血，缺氧部分并未显著降低。因此，白血病肝脏中的缺氧细胞和实体肿瘤中的缺氧细胞在诱导相对高氧血症后的氧合能力上有显著差异。
