4-氯-2-甲基苯氧基乙酸的细菌代谢。通过侧链裂解形成乙醛酸。
Bacterial metabolism of 4-chloro-2-methylphenoxyacetate. Formation of glyoxylate by side-chain cleavage.
作者信息
Gamar Y, Gaunt J K
出版信息
Biochem J. 1971 May;122(4):527-31. doi: 10.1042/bj1220527.
Abstract
Crude extracts of Pseudomonas sp. grown on 4-chloro-2-methylphenoxyacetate as sole source of carbon were shown to oxidize 4-chloro-2-methylphenoxyacetate to 5-chloro-o-cresol and glyoxylate. A labelled 2,4-dinitrophenylhydrazone was isolated from an incubation mixture in which 4-chloro-2-methylphenoxy[carboxy-(14)C]acetate was used. The hydrazone was shown to behave identically on thin-layer chromatograms with the authentic 2,4-dinitrophenylhydrazone of glyoxylate. Radioactivity assay showed that 82% of the side chain of 4-chloro-2-methylphenoxyacetate was recovered as glyoxylate.
摘要
以4-氯-2-甲基苯氧基乙酸作为唯一碳源生长的假单胞菌属细菌的粗提取物，被证明能将4-氯-2-甲基苯氧基乙酸氧化为5-氯邻甲酚和乙醛酸。在使用4-氯-2-甲基苯氧基[羧基-(14)C]乙酸的孵育混合物中分离出一种标记的2,4-二硝基苯腙。该腙在薄层色谱图上的行为与乙醛酸的 authentic 2,4-二硝基苯腙完全相同。放射性测定表明，4-氯-2-甲基苯氧基乙酸侧链的82% 以乙醛酸形式回收。
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