Gschnait F, Brenner W
J Invest Dermatol. 1979 Dec;73(6):566-9. doi: 10.1111/1523-1747.ep12541607.
Langerhans cells are considered to play an important role in the initiation of the immune response. This study was performed in order to analyze the kinetics of the Langerhans cell population under different experimental conditions. Using tritiated thymidine, the number of labeled Langerhans cells (demonstrated by the Leucinaminopeptidase reaction), and of labeled basal keratinocytes was investigated by autoradiography in guinea pig skin in vivo, before and 2, 5 and 8 days after stripping and before and 2, 5 and 8 days after initiation of repeated topical exposures to a 0.25% solution of dinitrochlorobenzene (DNCB). In addition the total number of Langerhans cells per mm2 was determined before and after DNCB treatment of epidermal guinea pig sheet preparations using the ATPase reaction. A total of more than 100,000 cell as of basal keratinocytes was stimulated significantly (by statistical analysis), both by stripping and by application of DNCB. After stripping, however, the increase of the Langerhans cell turnover was found to be secondary to the turnover of basal keratinocytes, whereas after DNCB application the increase in the proliferative activity of Langerhans cells appeared as the primary event in epidermal cellular kinetics.
朗格汉斯细胞被认为在免疫反应的启动中起重要作用。进行这项研究是为了分析在不同实验条件下朗格汉斯细胞群体的动力学。使用氚标记的胸腺嘧啶核苷，通过放射自显影术在豚鼠皮肤体内研究了标记的朗格汉斯细胞（通过亮氨酰氨肽酶反应显示）和标记的基底角质形成细胞的数量，分别在剥离前、剥离后2天、5天和8天以及开始反复局部暴露于0.25%二硝基氯苯（DNCB）溶液前、暴露后2天、5天和8天进行观察。此外，使用ATP酶反应测定了豚鼠表皮片制备物在DNCB处理前后每平方毫米朗格汉斯细胞的总数。总共超过100,000个基底角质形成细胞受到显著刺激（经统计分析），无论是通过剥离还是通过应用DNCB。然而，剥离后，发现朗格汉斯细胞更新的增加是基底角质形成细胞更新的继发结果，而应用DNCB后，朗格汉斯细胞增殖活性的增加似乎是表皮细胞动力学中的主要事件。
