Baker D, Parker D D, Turk J L
Br J Dermatol. 1985 Sep;113(3):285-94. doi: 10.1111/j.1365-2133.1985.tb02080.x.
Guinea pig skin was depleted of Langerhans cells (LC) as assessed by ATPase and Ia staining using several techniques. The LCs were depleted either by tape-stripping or exposure of the animals to UV-B or UV-C radiation. Guinea-pigs were sensitized to 2,4-dinitrochlorobenzene (DNCB) by application of the sensitizer to the epidermis depleted of LC. Minimally suppressed contact reactions were found in animals exposed to both wavelengths of radiation, but this was shown to be a systemic rather than a local effect. Tape-stripping did not alter the degree of contact sensitivity when guinea-pigs were sensitized with a large dose of DNCB. When a non-sensitizing dose of DNCB was applied to the ear depleted of LC by tape-stripping, contact sensitivity resulted. Although the depletion of LCs was 97% following UV-B, 93% with UV-C and 78% after tape-stripping, at no time were LCs completely absent from the epidermis.
通过几种技术进行的ATP酶和Ia染色评估显示，豚鼠皮肤中的朗格汉斯细胞（LC）已被耗尽。通过胶带剥离或使动物暴露于UV-B或UV-C辐射来耗尽LC。通过将敏化剂应用于已耗尽LC的表皮，使豚鼠对2,4-二硝基氯苯（DNCB）致敏。在暴露于两种辐射波长的动物中发现接触反应受到轻微抑制，但这被证明是一种全身性而非局部性效应。当用大剂量DNCB使豚鼠致敏时，胶带剥离并未改变接触敏感性的程度。当将非致敏剂量的DNCB应用于通过胶带剥离而耗尽LC的耳朵时，产生了接触敏感性。尽管UV-B照射后LC的耗尽率为97%，UV-C照射后为93%，胶带剥离后为78%，但表皮中LC从未完全缺失。
