Posner I, Bosch V
J Lipid Res. 1971 Nov;12(6):768-72.
A simple and specific method for assaying lipoprotein lipase activity is described. Postheparin plasma, heart homogenates, or extracts of acetone powder of adipose tissue were incubated with a triolein-coated Celite substrate, and enzyme activity was determined from the rate of free fatty acid (FFA) release in the incubation system. FFA release was linear for 30 min, and was proportional to protein concentration in the incubation system. FFA release was decreased by addition of deoxycholate or Triton X-100. Increasing the concentration of heparin in the incubation system caused a gradual decrease in FFA release by postheparin plasma and increases in activity of heart homogenates and adipose tissue lipoprotein lipase. The Celite substrate was found to be satisfactory for assaying pancreatic lipase activity as well.
本文描述了一种简单且特异的脂蛋白脂肪酶活性测定方法。将肝素后血浆、心脏匀浆或脂肪组织丙酮粉提取物与三油精包被的硅藻土底物一起孵育，通过孵育体系中游离脂肪酸（FFA）的释放速率来测定酶活性。FFA释放在30分钟内呈线性，且与孵育体系中的蛋白质浓度成正比。加入脱氧胆酸盐或 Triton X - 100可使FFA释放减少。增加孵育体系中肝素的浓度会导致肝素后血浆FFA释放逐渐减少，同时心脏匀浆和脂肪组织脂蛋白脂肪酶的活性增加。还发现硅藻土底物也适用于测定胰腺脂肪酶活性。
