Cernik A A, Sayers M H
Br J Ind Med. 1971 Oct;28(4):392-8. doi: 10.1136/oem.28.4.392.
392-398. The presence of lead in blood is the most incontrovertible evidence of absorption but hitherto the need for venepuncture has limited its determination in the supervision of industrial workers. Micro-methods using atomic absorption spectrophotometry (AAS) have, however, made possible the development of a sufficiently reliable test using a drop of blood obtainable by ear prick for use in the field for screening purposes. A micro-sampling method by AAS is compared with a routine polarographic procedure (POL) using venous blood (corr. coeff. = 0·990). The pipetting of microlitres of blood can be eliminated by spotting the blood onto filter paper, allowing it to dry in air, and then using a punched-out standard disc of dried blood for analysis instead. Correlation of this method (PD) with the micro-sampling technique (AAS) is good (r=0·981). The PD method using capillary blood also correlates acceptably with the micromethod using venous blood (r = 0·913). A pilot field study using capillary blood estimated by the PD technique showed that with this method blood can be collected by ear prick in factories for monitoring workers in the lead industry, thus eliminating the need for routine venepuncture.
392 - 398. 血液中铅的存在是吸收的最确凿证据，但迄今为止，由于需要静脉穿刺，限制了其在工业工人监测中的测定。然而，使用原子吸收分光光度法（AAS）的微量方法使得开发一种足够可靠的测试成为可能，该测试使用通过耳刺获得的一滴血液用于现场筛查目的。将AAS微量采样方法与使用静脉血的常规极谱法（POL）进行比较（相关系数 = 0·990）。通过将血液点在滤纸上，让其在空气中干燥，然后使用冲压出的干血标准圆盘进行分析，可以省去微升血液的移液操作。该方法（PD）与微量采样技术（AAS）的相关性良好（r = 0·981）。使用毛细血管血的PD方法与使用静脉血的微量方法也具有可接受的相关性（r = 0·913）。一项使用PD技术估算毛细血管血的现场试点研究表明，使用这种方法可以在工厂通过耳刺采集血液，用于监测铅行业的工人，从而无需进行常规静脉穿刺。
