Berridge M V
J Exp Med. 1979 Oct 1;150(4):977-86. doi: 10.1084/jem.150.4.977.
A new class of cell surface antigens are described which are expressed on cells within a particular differentiation pathway. These antigens, termed cell-lineage-specific antigens, are shown to be distinct from differentiation antigens and from histocompatibility antigens. The presence of these antigens was demonstrated by raising antisera against terminally differentiated hemopoietic cells such as platelets, thymocytes, and macrophages and showing cross-reaction with the pluripotent stem cells from which these cells were derived. Quantitative absorption studies of each antiserum showed the antigens to be largely cell-lineage-specific. For example, the anti-stem cell activity in anti-platelet serum was not absorbed out with thymocytes or macrophages from the same mouse strain but was removed by absorption with platelets. Absorption of each antiserum with nonhemopoietic mouse tissues such as brain, kidney, liver, and testis did not reduce anti-stem cell activity. Thus, each antiserum was shown to be tissue specific and species specific. Hemopoietic cells from mouse strains other than CBA absorbed out most of the anti-stem cell activity indicating cell-lineage-specific antigens to be common to the mouse strains tested.
描述了一类新的细胞表面抗原，它们在特定分化途径的细胞上表达。这些抗原被称为细胞谱系特异性抗原，已证明它们不同于分化抗原和组织相容性抗原。通过制备针对终末分化造血细胞（如血小板、胸腺细胞和巨噬细胞）的抗血清，并显示与这些细胞来源的多能干细胞发生交叉反应，证明了这些抗原的存在。对每种抗血清的定量吸收研究表明，这些抗原在很大程度上是细胞谱系特异性的。例如，抗血小板血清中的抗干细胞活性不会被来自同一小鼠品系的胸腺细胞或巨噬细胞吸收，但会被血小板吸收而去除。用非造血小鼠组织（如脑、肾、肝和睾丸）吸收每种抗血清不会降低抗干细胞活性。因此，每种抗血清都显示出组织特异性和物种特异性。除CBA外的其他小鼠品系的造血细胞吸收了大部分抗干细胞活性，表明细胞谱系特异性抗原在所测试的小鼠品系中是常见的。
