Harłozińska A, Potomski J, Lawinska B, Noworolska A, Richter R
Br J Cancer. 1982 Feb;45(2):194-200. doi: 10.1038/bjc.1982.33.
Discontinuous density-gradient centrifugation was used to separate chronic granulocytic leukaemia (CGL) cells in the chronic phase and blast crisis (BC) into fractions containing granulocytes in individual stages of maturation. The occurrence of the Fc IgG (FcR) and complement-component receptors (CR1 and CR2) in each fraction was estimated. It was established that, with increasing yields of mature granulocytes, the proportion of cells bearing Fc and C3 receptors increased. The most important finding was that the high- and low-receptor categories of CGL cells in chronic phase depended on the percentage of FcR+ cells. In the high-receptor CGL group, in addition to FcR, the proportion of CR1+ and CR2+ cells was also greater than in the low-receptor CGL group. Some differences in clinical course of both immunological CGL groups were observed.
采用不连续密度梯度离心法，将慢性粒细胞白血病（CGL）慢性期以及急变期（BC）的细胞分离成不同组分，每个组分含有处于不同成熟阶段的粒细胞。对每个组分中Fc IgG（FcR）和补体成分受体（CR1和CR2）的出现情况进行了评估。结果表明，随着成熟粒细胞产出量的增加，带有Fc和C3受体的细胞比例上升。最重要的发现是，慢性期CGL细胞高受体和低受体类别取决于FcR+细胞的百分比。在高受体CGL组中，除FcR外，CR1+和CR2+细胞的比例也高于低受体CGL组。观察到两个免疫CGL组在临床病程上存在一些差异。
