Fraser J K, Leahy M F, Berridge M V
Blood. 1986 Sep;68(3):762-9.
Several cell surface and cytoplasmic markers specific for the megakaryocyte-platelet lineage have been described. However, as yet, none of these has been shown to be expressed on cells earlier than the committed megakaryocyte progenitor, CFU-Meg. The present study was aimed at determining whether platelet lineage antigens could be detected on human pluripotential stem cells. Rabbit antiserum against human platelets (APS) was extensively absorbed with erythrocytes and either platelets, neutrophils, monocytes, or cells of the monocytic cell line U937. The anti-stem cell antibodies in each absorbed antiserum were determined using a complement-dependent cytotoxic assay for the pluripotential stem cell CFU-mix. Platelets alone removed anti-stem cell antibodies from APS. Absorption of APS with platelets from a patient with Glanzmann's thrombasthenia failed to remove the anti-stem cell activity, providing evidence for involvement of the platelet glycoprotein IIb/IIIa complex. Antiserum against purified glycoprotein IIb and against glycoprotein IIIa also recognized stem cells, resulting in reduced formation of mixed colonies. Absorption of these antisera with normal platelets removed the anti-stem cell activity, indicating that both IIb and IIIa are represented on stem cells. Hence, cell surface antigens specific for the stem cell-megakaryocyte-platelet pathway are expressed on the platelet glycoprotein IIb/IIIa complex.
已经描述了几种对巨核细胞 - 血小板谱系具有特异性的细胞表面和细胞质标志物。然而，迄今为止，这些标志物均未显示在比定向巨核细胞祖细胞CFU - Meg更早的细胞上表达。本研究旨在确定是否能在人类多能干细胞上检测到血小板谱系抗原。用红细胞以及血小板、中性粒细胞、单核细胞或单核细胞系U937的细胞对兔抗人血小板抗血清（APS）进行广泛吸收。使用针对多能干细胞CFU - mix的补体依赖性细胞毒性试验来确定每种吸收抗血清中的抗干细胞抗体。仅血小板就能从APS中去除抗干细胞抗体。用患有血小板无力症患者的血小板吸收APS未能去除抗干细胞活性，这为血小板糖蛋白IIb/IIIa复合物的参与提供了证据。针对纯化的糖蛋白IIb和糖蛋白IIIa的抗血清也识别干细胞，导致混合集落形成减少。用正常血小板吸收这些抗血清可去除抗干细胞活性，表明干细胞上同时存在IIb和IIIa。因此，干细胞 - 巨核细胞 - 血小板途径特异性的细胞表面抗原在血小板糖蛋白IIb/IIIa复合物上表达。
