Bonner T P
J Parasitol. 1979 Oct;65(5):745-50.
The ultrastructure of Nippostrongylus brasiliensis intestinal cells was examined in free-living, feeding second-stage larvae, infective, nonfeeding third-stage larvae, and parasitic, feeding third-stage larvae. The intestinal cells of second-stage larvae were characterized by a well-developed microvillar border, large numbers of ribosomes, Golgi complexes, rough endoplasmic reticulum, and nuclei with prominent nucleoli. The intestinal cells of infective, third-stage larvae had very few microvilli and the cells were extremely narrow. Few ribosomes, Golgi complexes, and little rough endoplasmic reticulum were present. Nuclei did not contain nucleoli. When worms were introduced into an in vitro culture system, development of intestinal cells began. By 36 hr, microvilli were well differentiated and the cysoplasm contained numerous ribosomes and Golgi complexes, and rough endoplasmic reticulum, mitochondria, and nucleoli were prominent. These morphological changes were related to changes in the physiology of Nippostrongylus brasiliensis which occur during development from a free-living to parasitic form.
对巴西日圆线虫肠道细胞的超微结构进行了检查，研究对象包括自由生活、进食的第二期幼虫、感染性、不进食的第三期幼虫以及寄生性、进食的第三期幼虫。第二期幼虫的肠道细胞具有发育良好的微绒毛边界、大量核糖体、高尔基体、粗面内质网以及带有明显核仁的细胞核。感染性第三期幼虫的肠道细胞微绒毛极少，细胞极其狭窄。核糖体、高尔基体很少，粗面内质网也很少。细胞核不含核仁。当将线虫引入体外培养系统时，肠道细胞开始发育。到36小时时，微绒毛已充分分化，细胞质中含有大量核糖体和高尔基体，粗面内质网、线粒体和核仁都很明显。这些形态学变化与巴西日圆线虫从自由生活形态发育为寄生形态过程中发生的生理变化有关。
