Munson J W, Papadimitriou D, DeLuca P P
J Pharm Sci. 1979 Oct;68(10):1333-5. doi: 10.1002/jps.2600681041.
Solutions of ampicillin, carbenicillin, methicillin, oxacillin, penicillin G, and cephalothin in 5% dextrose were analyzed by nickel(II)-catalyzed hydroxylaminolysis. The reactions of these antibiotics were complete within 20 min at room temperature. Under the analytical conditions, molar absorptivities of the ferric-hydroxamate complexes ranged from 830 to 1005 liters/mole/cm. Coefficients of variation for the analysis of these antibiotics in 5% dextrose were typically less than 3% at concentrations of 1 mg/ml. Oxacillin was analyzed by the same method in normal saline and/or lactated Ringer solutions. The method also was applied to the analysis of chloramphenicol in aqueous solutions. Only ampicillin showed a significant decrease in concentration in 48 hr.
采用镍（II）催化的羟胺分解法分析了氨苄西林、羧苄西林、甲氧西林、苯唑西林、青霉素G和头孢噻吩在5%葡萄糖溶液中的情况。这些抗生素的反应在室温下20分钟内完成。在分析条件下，高铁异羟肟酸络合物的摩尔吸光率在830至1005升/摩尔/厘米之间。在1毫克/毫升的浓度下，5%葡萄糖溶液中这些抗生素分析的变异系数通常小于3%。苯唑西林在生理盐水和/或乳酸林格氏液中采用相同方法进行分析。该方法也应用于水溶液中氯霉素的分析。只有氨苄西林在48小时内浓度有显著下降。
