Gujral Rajinder Singh, Haque Sk Manirul, Shanker Prem
Vardhman Chemtech Ltd, Nimbua, Dera Bassi, Mohali, Punjab, India.
Int J Biomed Sci. 2009 Dec;5(4):373-9.
A simple and sensitive spectrophotometric method was developed for the determination of Oxacillin sodium. The method was based on charge transfer complexation reaction of the drug with iodine in methanol - dichloromethane medium. The absorbance was measured at 365 nm against the reagent blank. Under optimized experimental conditions, Beer's law is obeyed in the concentration ranges 2-8 μg/ml for Oxacillin Sodium. The method was validated for specificity, linearity, precision, accuracy, limit of quantitation, robustness and ruggedness. The LOD and LOQ value were 0.39 and 1.18 μg/ml respectively. The method was successfully applied to the analysis of Oxacillin sodium in Human Urine samples with good accuracy and precision.
建立了一种简单灵敏的分光光度法测定苯唑西林钠。该方法基于药物与碘在甲醇 - 二氯甲烷介质中的电荷转移络合反应。以试剂空白为参比，在365nm处测定吸光度。在优化的实验条件下，苯唑西林钠在2 - 8μg/ml浓度范围内符合比尔定律。该方法在特异性、线性、精密度、准确度、定量限、稳健性和耐用性方面均得到验证。检测限和定量限分别为0.39和1.18μg/ml。该方法成功应用于人尿样中苯唑西林钠的分析，准确度和精密度良好。
